Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Dynamic profiling of histone Kcr and Kla during neural differentiation in P19 embryonal carcinoma cells

ABSTRACT: Dynamic profiling of histone Kcr and Kla during neural differentiation in P19 embryonal carcinoma cells

PROVIDER: PRJNA721095 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR14205820_1.fastq.gz	Fastqsanger.gz
	SRR14205820_2.fastq.gz	Fastqsanger.gz
	SRR14205821_1.fastq.gz	Fastqsanger.gz
	SRR14205821_2.fastq.gz	Fastqsanger.gz
	SRR14205822_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 56

Similar Datasets

Dynamic profiling of histone Kcr and Kla during neural differentiation in P19 embryonal carcinoma cells

Project description:To gain a deeper insight into dynamic changes of histone lysine crotonylation and lactylation during embryonic neurogenesis, ChIP-seq, ATAC-seq and RNA-seq were performed to analyze the genome-wide changes of histone Kcr and Kla in the P19 embryonal carcinoma cells.

2022-06-30 | GSE171832 | GEO

Profiling of H3K9cr and H3K9ac in the P19 embryonal carcinoma cells

Project description:To gain a deeper insight into dynamic changes of H3K9cr and H3K9ac during cell fate commitment, ChIP-seq was performed to analyze the genome-wide changes of H3K9cr and H3K9ac during neuroectodermal differentiation of the P19 embryonal carcinoma cells.

2022-04-17 | GSE200519 | GEO

Dynamic profiling of histone Kcr and Kla during neural differentiation in vivo

2022-06-30 | GSE171088 | GEO

Dynamic profiling of histone Kcr and Kla during neural differentiation in vivo

Project description:Dynamic profiling of histone Kcr and Kla during neural differentiation in vivo

| PRJNA718305 | ENA

Differential expression of glycosyltransferases in P19 mouse embryonal carcinoma cells with or without differentiation by retinoic acid

Project description:The project is aimed at understanding the role of the Lex-containing L5 carbohydrate epitope in the developmental process of neural induction. We plan to identify 1) the protein to which the L5 glycan is attached, 2) glycosyltransferases upregulated during neural induction, 3) the corresponding L5 carbohydrate binding protein (CBP), 4) the mechanism of interaction between L5 and its CBP, and 5) the spatiotemporal regulation of L5 and L5-CBP expression during neural induction. There is evidence, by Westernblot and immunofluorescence, that the expression of the Lewis-x-like L5 carbohydrate epitope shows dramatic differences in expression between undifferentiated cells and cells differentiated with retinoic acid. We wish to know which enzyme(s) involved in the biosynthetic pathway may be up/downregulated under these experimental conditions. Two classes of p19 mouse embryonal carcinoma cell RNA were analyzed: undifferentiated and differentiated with retinotic acid. Classes were prepared in triplicate for a total of 6 samples. All samples were hybridized to the custom designed CFG GLYCOv2 glycogene array.

2011-03-02 | E-GEOD-27596 | biostudies-arrayexpress

Distribution of chromatin features in mouse P19 embryonal carcinoma cells

Project description:Distribution of chromatin features in mouse P19 embryonal carcinoma cells

| PRJNA484121 | ENA

P19-atRA-kinetics

Project description:P19 embryonal carcinoma cells were treated with 1µM all trans retinoic acid and total RNA was collected at different time points

2013-06-30 | E-MEXP-3669 | biostudies-arrayexpress

Profiling of H3K9cr and H3K9ac in the P19 embryonal carcinoma cells

Project description:Profiling of H3K9cr and H3K9ac in the P19 embryonal carcinoma cells

| PRJNA824866 | ENA

Whole-genome gene expression level changes in P19.6 mouse embryonal carcinoma cells compared to P19.6 cells treated for 48 hours with all-trans retinoic acid

Project description:Enhancers are developmentally-controlled transcriptional regulatory regions whose activities are modulated through histone modifications or histone variant deposition. Here, we show by genome-wide mapping that the newly discovered DNA modification 5-hydroxymethylcytosine (5hmC) is dynamically associated with transcription factor binding to distal regulatory sites during neural differentiation of mouse P19 cells as well as during adipocyte differentiation of mouse 3T3-L1 cells. Functional annotation reveals that regions gaining 5hmC are associated with genes expressed either in neural tissues when P19 cells undergo neural differentiation or in adipose tissue when 3T3-L1 cells undergo adipocyte differentiation. Furthermore, distal regions gaining 5hmC together with H3K4me2 and H3K27ac in P19 cells behave as differentiation-dependent transcriptional enhancers. Identified regions are enriched in motifs for transcription factors regulating specific cell fates like Meis1 in P19 cells and PPARgamma in 3T3-L1 cells. Accordingly, a fraction of hydroxymethylated Meis1 sites were associated with a dynamic engagement of the 5mC hydroxylase Tet1. In addition, kinetic studies of cytosine hydroxymethylation of selected enhancers indicated that DNA hydroxymethylation is an early event of enhancer activation. Hence, acquisition of 5hmC in cell-specific distal regulatory regions may represent a major event of enhancer progression toward an active state and participate in selective activation of tissue-specific genes A 6-chip study aiming to characterize regulated genes in P19.6 mouse embryonal carcinoma cells following 48 hours treatment with 1M-BM-5M all-trans retinoic acid. RNAs were prepared from three independent triplicate experiments.

2012-06-03 | E-GEOD-27334 | biostudies-arrayexpress

Distribution of chromatin features in mouse P19 embryonal carcinoma cells

Project description:CHIP to investigate Hmgn2 binding in P19 undifferentiated and differentiated neuronal cells.

2018-08-03 | GSE118031 | GEO