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Profiling of H3K9cr and H3K9ac in the P19 embryonal carcinoma cells

ABSTRACT: Profiling of H3K9cr and H3K9ac in the P19 embryonal carcinoma cells

PROVIDER: PRJNA824866 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR18689049_1.fastq.gz	Fastqsanger.gz
	SRR18689049_2.fastq.gz	Fastqsanger.gz
	SRR18689050_1.fastq.gz	Fastqsanger.gz
	SRR18689050_2.fastq.gz	Fastqsanger.gz
	SRR18689051_1.fastq.gz	Fastqsanger.gz

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Profiling of H3K9cr and H3K9ac in the P19 embryonal carcinoma cells

Project description:To gain a deeper insight into dynamic changes of H3K9cr and H3K9ac during cell fate commitment, ChIP-seq was performed to analyze the genome-wide changes of H3K9cr and H3K9ac during neuroectodermal differentiation of the P19 embryonal carcinoma cells.

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Dynamic profiling of histone Kcr and Kla during neural differentiation in P19 embryonal carcinoma cells

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Dynamic profiling of histone Kcr and Kla during neural differentiation in P19 embryonal carcinoma cells

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P19-atRA-kinetics

Project description:P19 embryonal carcinoma cells were treated with 1µM all trans retinoic acid and total RNA was collected at different time points

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Distribution of chromatin features in mouse P19 embryonal carcinoma cells

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Differential expression of glycosyltransferases in P19 mouse embryonal carcinoma cells with or without differentiation by retinoic acid

Project description:The project is aimed at understanding the role of the Lex-containing L5 carbohydrate epitope in the developmental process of neural induction. We plan to identify 1) the protein to which the L5 glycan is attached, 2) glycosyltransferases upregulated during neural induction, 3) the corresponding L5 carbohydrate binding protein (CBP), 4) the mechanism of interaction between L5 and its CBP, and 5) the spatiotemporal regulation of L5 and L5-CBP expression during neural induction. There is evidence, by Westernblot and immunofluorescence, that the expression of the Lewis-x-like L5 carbohydrate epitope shows dramatic differences in expression between undifferentiated cells and cells differentiated with retinoic acid. We wish to know which enzyme(s) involved in the biosynthetic pathway may be up/downregulated under these experimental conditions. Two classes of p19 mouse embryonal carcinoma cell RNA were analyzed: undifferentiated and differentiated with retinotic acid. Classes were prepared in triplicate for a total of 6 samples. All samples were hybridized to the custom designed CFG GLYCOv2 glycogene array.

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Project description:Gene expression profiles of undifferentiated mouse embryonal carcinoma cell strains (P19, P19CL6, and 4 P19CL6 sublines) were obtained, using Affymetrix GeneChip Mouse Genome 430A and 430B. Heart diseases such as cardiac infarction damage cardiomyocytes and consequently lead to significant loss of the contractile capacity of the heart. To repair functions of the injured heart, a great deal of research has attempted to develop regenerative medicine using pluripotent stem cell-based cardiomyocytes as cell therapy products. However, the efficiency of the current methods available for the cardiac differentiation of stem cells is insufficient for clinical settings. A comprehensive understanding of the mechanism involved in the cardiac differentiation of stem cells is necessary to improve the differentiation efficiency. To identify genes assosiated with cardiomyogenic potential, we isolated P19CL6 cell sublines possessing distinct properties in cardiomyogenesis and comprared their transcriptome profiles with those of mouse embryonal carcinoma P19 and P19CL6 cells. Total RNA isolated from undifferentiated EC cell strains (P19 cells, P19CL6 cells, and 4 P19CL6 sublines) using Affymetrix chips MOE430A and MOE430AB. CEL files unavailable.

2011-01-26 | E-GEOD-26875 | biostudies-arrayexpress

Gene expression data from undifferentiated mouse embryonal carcinoma cell strains (MOE430A and MOE430B)

2011-01-26 | GSE26875 | GEO

P19 embryonic carcinoma (EC) cells induced to form cardiomyocytes in vitro

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