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ABSTRACT: Transcriptome of Bombyx mori prothoracic gland

PROVIDER: PRJNA723919 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR14306779_1.fastq.gz	Fastqsanger.gz
	SRR14306779_2.fastq.gz	Fastqsanger.gz
	SRR14306780_1.fastq.gz	Fastqsanger.gz
	SRR14306780_2.fastq.gz	Fastqsanger.gz

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Proteomic analysis of Bombyx mori prothoracic glands from day 0 and day 6 of the 5th instar

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Genome-wide transcriptional analysis of PTTH-stimulated Bombyx prothoracic gland

Project description:This is part of a cross-species approach to characterize transcriptional response of the insect prothoracic gland (PG) to the prothoracicotropic hormone (PTTH). Genome-wide response of the PG to PTTH was analyzed in vitro in Bombyx and in vivo in Drosophila, and the results were compared to identify common components in the PTTH signaling pathway. Bombyx PGs were incubated in vitro for 1 or 3 hours with or without 10 nM recombinant PTTH. 12-18 larvae were used to prepare each RNA sample, and the experiment was conducted twice. Total RNA samples from two independent preparations were combined to obtain a single RNA sample for each treatment, which was subjected to RNA-seq.

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Bombyx mori Posterior and Median Silk Gland SAGE transcriptome

Project description:To identify functions that distinguish the posterior and median cells producing fibroin and sericin in the silk gland of Bombyx mori, serial analysis of gene expression (SAGE) profiles from both silk gland regions were analyzed and compared. The construction of a B. mori reference tag collection extracted from a set of 38000 Bombyx EST sequenced from the 3’ side, helped annotating the SAGE libraries. Most of the tags appeared at similar relative concentration in the two libraries except for those corresponding to silk proteins that were found region-specific and highly abundant. Strikingly, besides tags from silk protein mRNAs, 19 tags were found in the class of high abundance in the median cell library, which were absent in the posterior cell tag collection. Except tags from SP1 mRNA, no PSG specific tags were found in the same class of abundance. The analysis of MSG-specific different transcripts led to suggest that middle silk gland cell realizes more diversified functions as those already known, of synthesis and secretion of the silk sericins.

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