Project description:In order to further explore the expression of lncrna in asthma, we used lipopolysaccharide (LPS) to activate human monocyte macrophage THP-1, and then used deep sequencing method to detect the expression of lncrna in activated and inactive THP1 cells. The results showed that there were different expression of lncrna in LPS activated and inactive THP1 cells
Project description:To screen the genes and pathways regulated by BRD3 in the THP1 monocytes induced by LPS, THP1 monocytes were transfected by BRD3shRNA and then treated by LPS for 3 hours.
Project description:To screen the genes and pathways regulated by CREB1 in the THP1 monocytes induced by LPS, THP1 monocytes were transfected by CREB1 shRNA and then treated by LPS for 3 hours.
Project description:To screen the genes regulated by BRD3 in the THP1 monocytes induced by LPS, THP1 monocytes were treated by LPS for 1 hour in the presence or absence of OTX015
Project description:Transcriptome analysis of the human monocytic cell line THP1 (ATCC TIB-202) treated or not with phorbol myristate acetate (PMA) +/- lipopolysaccharides-lipopolysaccharides binding protein (LPS-LBP)
Project description:RNA-seq was performed to identify differences in the transcriptome between wildtype, CDK2-knockdown and JUN-knockdown THP1 cells treated with LPS against untreated controls.
Project description:microRNA expression profile in 5 indepent sets of paired samples of LPS+IL-4 activated versus non activated primary mouse spleen B cells Keywords: cell type comparison
Project description:microRNA expression profile in 5 indepent sets of paired samples of LPS+IL-4 activated versus non activated primary mouse spleen B cells Keywords: cell type comparison Activated B cells for miRNA array analysis were obtained after CFSE labelling (Molecular Probes), LPS+IL4 stimulation for 3 days and sorting of cells that had undergone at least 1 cell division. Sortings were performed with a FACSAria cell sorter (BD Biosciences).