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Gene expression in splenic B cells or thymocytes from mice with Nsd2 was edited

ABSTRACT: Gene expression in splenic B cells or thymocytes from mice with Nsd2 was edited

PROVIDER: PRJNA734787 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR14714583_1.fastq.gz	Fastqsanger.gz
	SRR14714583_2.fastq.gz	Fastqsanger.gz
	SRR14714584_1.fastq.gz	Fastqsanger.gz
	SRR14714584_2.fastq.gz	Fastqsanger.gz
	SRR14714585_1.fastq.gz	Fastqsanger.gz

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Gene expression in splenic B cells or thymocytes from mice with Nsd2 was edited

Project description:We performed RNA-Seq on primary splenic B cells and thymocytes that were collected from wild type and Nsd2-edited mice.

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Genome-wide maps of chromatin state in thymocytes from Nsd2-edited mice

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Genome-wide maps of chromatin state in thymocytes from Nsd2-edited mice

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Epigenome analysis of wild type and Nsd2 gene-edited mice

Project description:Genome wide DNA methylation profiling of thymocytes from wild type and Nsd2 knock out (KO) and Nsd2 knock in (KI). The 906th Proline of Nsd2 was substituted to Leucine in Nsd2 KI by gene editing. The Illumina Infinium Mouse methylation Beadchip was used to obtain DNA methylation profiles across approximately 270,000 CpGs in thymocytes. Samples included 2 wild type, 2 heterozygous knock out, 1 heterozygous knock in, and 1 homozygous knock in.

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Epigenome analysis of wild type and Nsd2 gene-edited mice

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Project description:Themis1, a recently described T-lineage specific protein, is essential for thymic positive and negative selection. Although Themis1 has been clearly identified as a component of the T cell antigen receptor (TCR) signalosome, its precise role in TCR signaling remains unclear. Here, we used quantitative proteomic and TCR signaling reporter mice to gain insight into Themis1 signaling function. Mass spectrometry analysis of the Themis1 interactome identified Grb2, SHP1 and Vav1 as the principal interacting partners of Themis1 in thymocytes. The dataset contains mass spectrometry results from the analysis of 6 different kind of AP-MS purifications (based on immunoprecipitation using a Themis1 antibody) starting from the following samples: - thymocytes from WT mice, non stimulated (noted WT NS) - thymocytes from WT mice, stimulated with pervanadate (noted WT P) - thymocytes from GRB2 +/- mice (with decreased expression of GRB2), non stimulated (noted GRB2 NS) - thymocytes from GRB2 +/- mice (with decreased expression of GRB2), stimulated with pervanadate (noted GRB2 P) - thymocytes from Themis1 -/- mice (knock-out for Themis1), non stimulated (noted KO NS) - thymocytes from Themis1 -/- mice (knock-out for Themis1), stimulated with pervanadate (noted KO P) Three biological replicates were prepared for these 6 different conditions (noted, 1,2,3), yielding 18 analyzed samples. Three technical nanoLC-MS runs were acquired for each sample (noted R1, R2, R3), leading to the 54 nanoLC-MS raw files contained in the dataset.

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Defining the NSD2 interactome: PARP1 PARylation reduces NSD2 histone methyltransferase activity and impedes chromatin binding

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