Project description:ChIP-seq for Drosophila Insensitive, together with IgG control. Chromatin extracted from 2.5-6h w[1118] embryos and 6.5-12h w[1118] embryos.
Project description:To determine what genes are affect to the reduction in mutation frequency by 500µGy gamma irradiation of Drosophila melanogaster. The strain is w[1118] (a loss of function mutant of compound eye pigment gene white with Canton-S background). Keywords: time course
Project description:The larval ovary is made up of multiple cell types including germ cells and somatic cells. The diversity of cell types and transcriptional regulation is not fully understood. To get single cell resolution of larval ovary regulation, we generated single-cell RNA expression profiles (scRNA-Seq) from late third instar larval ovaries of a reference Drosophila melanogaster genotype w[1118].
Project description:MicroRNAs detected in Drosophila melanogaster unfertilized eggs Bloomington w[1118] flies were kept at 25ºC on cornmeal based media, with 12 hours light/dark cycles. Virgin females were sorted at the pupae stage to avoid any unwanted fertilization. In a population cage I let 80-100 females to lay eggs in apple juice agar plates for 8 hours, collecting 1 hour after dawn. Eggs were collected with a sieve and washed with saline solution. Small RNA was size selected and sequenced.
Project description:Thermal acclimation study on Drosophila melanogaster reared at 3 different temperatures (12, 25, and 31oC). The proteomic profiles of D. melanogaster under these different temperatures were analyzed and compared using label-free tandem mass spectrometry.