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RNA-sequencing of Vg4+Vd4+gdT cells isolated from skin-draining lymph nodes of WT and NF-kB1 deficient mice after imiquiod treatment

ABSTRACT: RNA-sequencing of Vg4+Vd4+gdT cells isolated from skin-draining lymph nodes of WT and NF-kB1 deficient mice after imiquiod treatment

PROVIDER: PRJNA741275 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR14908058.fastq.gz	Fastqsanger.gz
	SRR14908059.fastq.gz	Fastqsanger.gz
	SRR14908060.fastq.gz	Fastqsanger.gz
	SRR14908061.fastq.gz	Fastqsanger.gz
	SRR14908062.fastq.gz	Fastqsanger.gz

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RNA-sequencing of Vg4+Vd4+gdT cells isolated from skin-draining lymph nodes of WT and NF-kB1 deficient mice after imiquiod treatment

Project description:Purpose: The numbers of Vg4+Vd4+gdT17 cells in skin-draining lymph nodes were significantly reduced in imiqimod-treated NF-kB1-/- mice compared to those in IMQ-treated WT mice. To address the mechanism of NF-kB1-mediated proliferation of Vg4+Vd4+gdT17 cells, we performed RNA-sequencing of Vg4+Vd4+gdT cells isolated from the draining lymph nodes. Method: Mice were treated daily with 50 mg of 5% imiquimod cream or sham cream on both ears for 4 days. Single cell suspensions were separated from cervical lymph nodes. Vg4+Vd4+gdT cells were isolated by a cell sorter. N=3 each. Results: Using an optimized data analysis workflow, 552 of the transcripts showed differential expression between the WT and NF-kB1 deficient mice after imiquiod treatment, with a fold change ≥2 and p value <0.05.

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