Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Studying human embryonic stem cell reveals molecular mechanisms of cold resistance

ABSTRACT: Studying human embryonic stem cell reveals molecular mechanisms of cold resistance

PROVIDER: PRJNA743312 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Studying human embryonic stem cell reveals molecular mechanisms of cold resistance

Project description:we investigated transcriptomes of H1 ESCs in the response to cold treatment.

2024-03-01 | GSE179344 | GEO

Studying iPSCs from a hibernating mammal reveals molecular mechanisms of cold resistance in neural tissues

Project description:Studying iPSCs from a hibernating mammal reveals molecular mechanisms of cold resistance in neural tissues

| PRJNA362861 | ENA

Studying iPSCs from a hibernating mammal reveals molecular mechanisms of cold resistance in neural tissues

Project description:To explore the cold resistant feature of ground squirrel neuron cells, we compared transcriptomes of human and ground squirrel iPSC derived neurons in the response to cold treatment. We identified several key pathways that are critical for the maintenance of neuronal microtubule cytoskeleton integrity at low temperature.

2018-03-22 | GSE93935 | GEO

Molecular regulation mechanism of cold acclimation and cold tolerance in tea leaves

Project description:Recently, intensive global climate change has become a major factor impacting plant survival during the winter. Freezing cold temperatures during the winter and abnormal temperature fluctuations during the winter and early spring are the most harmful ambient factors threatening tea plant winter survival and currently cause marked economic losses in tea production. In this study, by simulating natural climate change, we established cold acclimation (CA) and rapid cold stress (after CA) conditions to comprehensively investigate the transcriptome changes involved in CA and rapid cold stress. Electrolyte leakage (EL) rate and expression profile clustering analyses confirmed that the experimental design was valid. Comparative transcription analysis identified many differentially expressed genes (DEGs) involved in both processes. Time course and pathway enrichment analyses further revealed the physiological changes that occur during the initial period of CA and the cell wall changes that occur throughout the entire CA process; these changes play crucial roles in increasing freezing tolerance during this process. Compared with CA, different cold response mechanisms were rapidly activated under cold stress; however, the subsequent accumulation of reactive oxygen species, which affect multiple aspects, caused by freezing cold could be the harshest factor impairing tea leaves. Moreover, we investigated 60 DEGs shared by both processes and highlighted the importance of KCSs, HXXXD-type acyl-transferase family proteins, NAC080, SWEETs and ENOs in the responses to various cold conditions. These results greatly improve our knowledge of cold response mechanisms in tea plants and provide meaningful information for functional studies investigating cold tolerance-related genes.

2017-09-29 | GSE104390 | GEO

Deep RNAseq indicates protective mechanisms of cold-tolerant indica rice plants during early vegetative stage

Project description:Low temperature exposure during early vegetative stages limits rice plant’s growth and development. Most genes previously related to cold tolerance in rice are from the japonica subspecies. To help clarify the mechanisms that regulate cold tolerance in young indica rice plants, comparative transcriptome analysis of 6 h cold-treated leaves from two genotypes, cold-tolerant and cold-sensitive, was performed. The cold-tolerant and cold-sensitive genotypes were previously characterized, and are sister lines (derived from the same crossing).

2017-09-06 | GSE103480 | GEO

A Human Embryonic Stem Cell Reporter Line for Studying Cardiomyocyte Biology

Project description:A Human Embryonic Stem Cell Reporter Line for Studying Cardiomyocyte Biology

| PRJNA436718 | ENA

Studying heterogeneity in embryonic stem cells using the approach of search-engine

Project description:Studying heterogeneity in embryonic stem cells using the approach of search-engine

| PRJNA792553 | ENA

Transcriptome and metabolome changes in Chinese cedar during cold acclimation reveal the roles of flavonoids in needle discoloration and cold resistance

Project description:Cryptomeria fortunei growth and development are usually affected by low temperatures. Despite the evergreen nature of this species, most needles turn yellowish-brown in cold winters. The underlying discoloration mechanisms that cause this phenomenon in response to cold acclimation remain poorly understood. Here, we measured the pigment content and ultrastructure of normal wild-type (Wt) and evergreen mutant (GM) C. fortunei needles and performed integrated transcriptomic and metabolomic analyses to explore potential discoloration mechanisms. The results showed that the needle chlorophyll content of these two genotypes decreased in winter. Wt needles showed greater decrease in the chlorophyll content and local destruction of chloroplast ultrastructure, and contained larger amounts of flavonoids than GM needles, as shown by metabolomics analysis. We subsequently identified key differentially expressed genes in the flavonoid biosynthesis pathway and observed significantly upregulated flavonol synthase (FLS) expression in Wt needles compared to GM needles, that significantly increased the anthoxanthin (flavones and flavonols) content, which is likely a key factor underlying the difference in needle color between these two genotypes. Therefore, flavonoid metabolism may play important roles in the cold resistance and needle discoloration of C. fortunei, and our results provide an excellent foundation for the molecular mechanism of C. fortunei in response to cold stress.

2022-08-12 | MTBLS3184 | MetaboLights

Characterization of biological pathways regulating acute cold resistance of zebrafish - experiment 2

Project description:This experiment was performed to investigate the functional mechanisms of the critical pathways including ERK, p38 MAPK and the transcription factor Foxo1 in enhancing cold resistance of zebrafish.Specific inhibitors of these pathways were used to treat zebrafish larvae under both normal and low temperatures to investigate the functions of these pathways in regulating cold stress response.

2021-03-06 | GSE168356 | GEO

Large scale phosphoprotein profiling to unravel Drosophila cold acclimation regulatory mechanisms

Project description:In this study we compared the phosphoproteome of control and cold acclimated Drosophila melanogaster in order to unravel the cold acclimation regulation mechanisms. For this prurpose we enriched phosphopeptides from each sample using a sequential elution from IMAC strategy (SIMAC) and analyzed them with a LTQ-OrbitrapXL mass spectrometer using a combination of different fragmentation strategies (classical data dependant analysis using CID, neutral loss scanning and multi-stage activation) in order to improve the number of identified phosphopeptides.

2017-05-16 | PXD005311 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data