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ABSTRACT: Molecular markers discovery in diverse genotypes and lines of Indian Black pepper species (Piper nigrum)

PROVIDER: PRJNA744363 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR15057622_1.fastq.gz	Fastqsanger.gz
	SRR15057622_2.fastq.gz	Fastqsanger.gz
	SRR15057623_1.fastq.gz	Fastqsanger.gz
	SRR15057623_2.fastq.gz	Fastqsanger.gz
	SRR15057624_1.fastq.gz	Fastqsanger.gz

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Black pepper proteome - Transcriptome- Assisted Label-Free Quantitative Proteomics Analysis Reveals Novel Insights into Piper nigrum-Phytophthora capsici Phytopathosystem

Project description:In this study, we have evaluated the proteomic changes that occur in Piper nigrum L.(black pepper) after infection by the pathogen Phytophthora capsici. We report novel leaf proteins from black pepper identified by an integrated transcriptome-assisted label-free quantitative proteomics pipeline. Several previously described methods were used to create this data set. Detached leaves were inoculated with either mock treatment, or the oomycete pathogen and small tissue samples only around the site of inoculation were collected for protein sample preparations. In order to quantify protein abundance in the samples being compared, we used a label free method of spiking samples with a known ratio of pre-digested peptide samples to normalize endogenous protein abundance in the MS detection. Our study attempts to explain the basal immune components of black pepper when challenged with P. capsici.

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Project description:The purpose of this experiment was to assess the effect of a synergistic combination of natural pyrethrin and an ethylacetate extract of Piper nigrum seeds (a botanical insecticide). This effect was compared to the effects of P. nigrum or pyrethrin used alone. Due to the synergistic nature of the mixture, it was predicted that gene expression profiles in this treatment would reflect this effect. Keywords: insecticide response, stress-response

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