Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Drosophila melanogaster

ABSTRACT: Deubiquitinase Nonstop regulates apoptosis by controlling the transcription of apoptosis related genes during Drosophila eye development

PROVIDER: PRJNA745486 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR15112646_1.fastq.gz	Fastqsanger.gz
	SRR15112646_2.fastq.gz	Fastqsanger.gz
	SRR15111747_1.fastq.gz	Fastqsanger.gz
	SRR15111747_2.fastq.gz	Fastqsanger.gz
	SRR15112647_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 24

Similar Datasets

Eye proteome of Drosophila melanogaster

Project description:We report the proteome composition of the Drosophila eye – a compound organ that is highly enriched in membrane proteins. The fly eye is a popular model to study the physiology of vision by means of genetic, pharmacological, and dietary interference.While the eye transcriptome and development-related changes of gene expression profiles have been extensively studied, little is known about the eye proteome.we employed GeLC-MS/MS to identify and rank the abundances of 3516 eye proteins. Moreover, we applied our MS Western method to determine the absolute (molar) abundances of a related set of proteins that are important for photoreceptor structure (including maintenance) and function (phototransduction). Altogether, we provide a comprehensive and expandable proteomics resource that will be valuable for a variety of studies of ocular biochemistry, physiology, and development.

2024-08-09 | PXD044845 | Pride

Drosophila melanogaster

Project description:Chromatin Remodeler Dmp18 regulates apoptosis by controlling H2Av incorporation into pro-apoptotic genes in Drosophila imaginal disc development

| PRJNA761186 | ENA

RNA-seq identifies genes that are regulated by SAGA deubiquitinase activity in glial nuclei of the Drosophila central nervous system during larval development

Project description:Purpose: Next-generation sequencing (NGS) was used to elucidate genes in Drosophila visual system glia that are sensitive to impairment of the deubiquitinase activity of the Spt-Ada-Gcn5 Acetyltransferase (SAGA) complex. Methods: The nuclear envelope of glial cells was genetically labled with a KASH-GFP fusion protein using the Gal4/UAS binary expression system and repo-Gal4 as driver in wild-type, nonstop and sgf11 third instar larvae. GFP-labeled glial nuclei were released by homogenization and then isolated from the central nervous system/eye antennal disc complex of Drosophila melanogaster third instar larvae from each genotype, and mRNA was extracted. For RNA-seq, dsDNA was generated using the Ovation RNA-Seq System V2 (Nugen technologies), DNA libraries were generated using standard methods (Illumina), and paired-end 100-base fragements were sequenced on an Illumina HiSeq2500 next generation sequencer. Result: EdgeR analysis with significance cut-off of FDR<0.01 identified 629 genes that are significantly downregulated, and 779 genes that are significantly upregulated, in both the nonstop and sgf11 visual system glia as compared to wild-type glia.

2016-06-09 | GSE75681 | GEO

Drosophila melanogaster

Project description:Drosophila Eye Development - Larval Stage

| PRJNA84699 | ENA

Drosophila melanogaster

Project description:Expression data from transdetermined Drosophila eye antennal imaginal disc by winged eye overexpression.

| PRJNA345425 | ENA

Drosophila melanogaster

Project description:Expression data from third instar Drosophila eye discs

| PRJNA131911 | ENA

Drosophila melanogaster

Project description:Eye imaginal disc expressing transgenic human proinsulin in Drosophila eye under regulation of GMR-GAL4

| PRJNA184672 | ENA

RNA Seq analysis of 2.5 hour embryos of Drosophila melanogaster in the absence of Ataxin-7 or non-stop.

Project description:The Spt-Ada-Gcn5-acetyltransferase (SAGA) chromatin-modifying complex is a transcriptional coactivator that contains four different modules of subunits. The intact SAGA complex has been well characterized for its function in transcription regulation and development. However, little is known about the roles of individual modules within SAGA and if they have any SAGA independent functions. Here we demonstrate that the two enzymatic modules of Drosophila SAGA are differently required in oogenesis. Ada2b is part of the HAT module (Histone Acetyl Transferase), whereas Ataxin-7 and non-stop are members of the DUB module (Deubiquitinase). Loss of the HAT activity blocks oogenesis, while loss of the DUB activity does not. However, the DUB module regulates a subset of genes in early embryogenesis and loss of the DUB subunits causes defects in embryogenesis. ChIP-seq analysis of ada2b, spt3, nonstop, and sgf11revealed that both the DUB and HAT modules bind most SAGA target genes even though many of these targets do not require the DUB module for expression. Furthermore, we found that the DUB module can bind to chromatin and regulate transcription independently of the rest of SAGA. Our results suggest that the DUB module has functions within SAGA as well as independent functions.

2017-10-01 | GSE98863 | GEO

RNA Seq analysis of whole ovaries following mutation of SAGA subunits: Ada2b, Ataxin-7, or non-stop in Drosophila melanogaster

2017-10-01 | GSE98864 | GEO

Drosophila melanogaster

Project description:Expression data from Drosophila third instar larvae eye imaginal discs

| PRJNA152937 | ENA