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Single-cell transcriptomics unveils xylem cell development and evolution

ABSTRACT: Single-cell transcriptomics unveils xylem cell development and evolution

PROVIDER: PRJNA746591 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR15142072.fastq.gz	Fastqsanger.gz
	SRR15142073_1.fastq.gz	Fastqsanger.gz
	SRR15142073_2.fastq.gz	Fastqsanger.gz
	SRR15142074.fastq.gz	Fastqsanger.gz
	SRR15142074_1.fastq.gz	Fastqsanger.gz

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Single-cell transcriptomics unveils xylem cell development and evolution

Project description:The plant xylem tissue is responsible for water transportation, mechanical support and lateral growth, and can be utilized as wood. The seed plant xylem has a radial system composed of ray parenchyma cells and an axial system of fusiform cells, including tracheids in gymnosperms and vessel elements and libriform fibers in angiosperms. Little is known about the developmental programs and evolutionary relationships of these xylem cell types. Here we show that the radial and axial systems of stem-differentiating xylem are differentially conserved across four divergent woody angiosperms. Through transcriptomic profiling of single cells and cell-type regions isolated by laser capture microdissection, we identified cell types corresponding to single-cell clusters in Populus trichocarpa. Cross-species analyses of single-cell trajectories demonstrated highly conserved development in ray lineages across angiosperms. While the core eudicots P. trichocarpa and Eucalyptus grandis share nearly identical fusiform cell lineages, the tracheids in the basal eudicot Trochodendron aralioides, an evolutionarily reversed character, exhibited an expression pattern highly similar to that of vessel elements. It suggests that water transportation, instead of mechanical support, is the dominant feature of tracheary elements. Furthermore, we found that the more basal angiosperm Liriodendron chinense has a distinct fusiform lineage. This evo-developmental framework of xylem cell lineages provides a comprehensive understanding of the formation of the most abundant tissue on Earth.

2022-12-25 | GSE180121 | GEO

Single-cell transcriptomics unveils xylem cell development and evolution.

Project description:BackgroundXylem, the most abundant tissue on Earth, is responsible for lateral growth in plants. Typical xylem has a radial system composed of ray parenchyma cells and an axial system of fusiform cells. In most angiosperms, fusiform cells comprise vessel elements for water transportation and libriform fibers for mechanical support, while both functions are performed by tracheids in other vascular plants such as gymnosperms. Little is known about the developmental programs and evolutionary relationships of these xylem cell types.ResultsThrough both single-cell and laser capture microdissection transcriptomic profiling, we determine the developmental lineages of ray and fusiform cells in stem-differentiating xylem across four divergent woody angiosperms. Based on cross-species analyses of single-cell clusters and overlapping trajectories, we reveal highly conserved ray, yet variable fusiform, lineages across angiosperms. Core eudicots Populus trichocarpa and Eucalyptus grandis share nearly identical fusiform lineages, whereas the more basal angiosperm Liriodendron chinense has a fusiform lineage distinct from that in core eudicots. The tracheids in the basal eudicot Trochodendron aralioides, an evolutionarily reversed trait, exhibit strong transcriptomic similarity to vessel elements rather than libriform fibers.ConclusionsThis evo-devo framework provides a comprehensive understanding of the formation of xylem cell lineages across multiple plant species spanning over a hundred million years of evolutionary history.

| S-EPMC9830878 | biostudies-literature

Transcriptomics of xylem from a Populus deltoides population

Project description:Transcriptomics of xylem from a Populus deltoides population

| PRJNA589026 | ENA

Compression Xylem vs. Opposite Xylem

Project description:Compression Xylem vs. Opposite Xylem

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Compression Xylem vs. Opposite Xylem

Project description:This SuperSeries is composed of the following subset Series: GSE37678: cDNA Microarray 1: Compression Xylem vs. Opposite Xylem GSE37736: cDNA Microarray 2: Compression Xylem vs. Opposite Xylem Refer to individual Series

2012-05-05 | E-GEOD-37789 | biostudies-arrayexpress

Transcriptomics of xylem from a Populus deltoides population

Project description:We sequenced the xylem transcriptome from a population of unrelated individuals of P. deltoides to uncover a master regulator of lignin biosynthesis. Grant ID: IOS-1444543 Grant Title: Genome and transcriptome based prediction, and regulator inference, o molecular and whole-plant phenotypes. Funding source: NSF Plant Genome Research Program.

2020-05-04 | GSE140232 | GEO

Integrative single-cell transcriptomics clarifies adult neurogenesis and macroglia evolution

Project description:Integrative single-cell transcriptomics clarifies adult neurogenesis and macroglia evolution

| PRJNA937732 | ENA

Expression data from two year old newly formed developing xylem and lignified xylem of Populus tomentosa

Project description:We take the two year old plant for sampling. Use the Affymetrix poplar gene chip to elucidate the gene functions and mechanisms in Populus tomentosa newly formed developing xylem and lignified xylem. We used microarrays to detail the global programme of gene expression in newly formed developing xylem and lignified xylem.

2013-05-16 | GSE46946 | GEO

Control of plasma membrane-associated actin polymerization specifies the pattern of the cell wall in xylem vessels.

Project description:Cell wall patterning is central to determining the shape and function of plant cells. Protoxylem and metaxylem vessel cells deposit banded and pitted cell walls, respectively, which enable their distinctive water transport capabilities, but the mechanisms that specify which cell wall pattern is laid down remain unknown. Here, we show that the pitted cell wall pattern in metaxylem vessels is specified by transcriptional control of actin polymerization. A newly isolated allele of KNOTTED-like homeobox transcription factor 7 (KNAT7) was associated with the formation of banded cell walls in metaxylem vessels. To identify the genes responsible for altering the cell wall pattern in knat7 xylem vessels, we performed an RNA-seq analysis of knat7. We used in vitro cultures of hypocotyls and cotyledons because the population of differentiating xylem cells in intact roots is tiny and these methods efficiently induce ectopic differentiation of xylem vessel elements. Formin Homology Domain-Containing Protein 11 (FH11) was upregulated in knat7 metaxylem, but expressed only in protoxylem vessels in wild-type plants. Loss of FH11 suppressed the knat7 phenotype. Conversely, ectopic overexpression of FH11 in metaxylems mimicked the knat7 phenotype. FH11 function required its plasma membrane anchoring and the actin polymerization activity. Actin polymerization by FH11 at the plasma membrane caused the dispersion of activated ROP GTPases, the determinant of pit formation. These results suggest that decontrol of actin polymerization at the plasma membrane abolishes the pitted cell wall formation and promotes the formation of banded cell walls in metaxylem vessels. This study unveils the importance of the proper control of actin polymerization on cell wall pattern specification.

2025-01-15 | GSE286145 | GEO

Transcriptomics of Xanthomonas campestris pv campestris in cabbage xylem sap

Project description:Xylem sap of young cabbage plantlets was recovered from root pressure exudation and used as a growth medium for the vascular pathogen Xanthomonas campestris pv campestris, the causative agent of the black rot of Brassicaceae.

2014-01-04 | E-MTAB-2290 | biostudies-arrayexpress

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