Project description:To assess DNA accessbility in adult Gabra3 knockout mouse retinas, especially at the Gabra1 promoter, we ultilized the Assay for Transposase Accessible Chromatin (ATAC-Seq; Buenrostro et al., 2013) on whole retinas from Gabra3 knockout mice and littermate controls. We observed no significant differences between WT and KO retinas with respect to DNA accessibility.
Project description:Nhlh2 is a neuronal helix-loop-helix transcription factor. The goal of this study was to determine the pattern of gene expression in wildtype mouse retina and to compare it to that in the retinas of mice in which the nhlh2 gene had been knocked out. RNA was extracted from three pairs of littermates, each comprising one wildtype and one knockout sample. The littermate samples are: retina_wt_rep1 & retina_nhlh2_rep1; retina_wt_rep2 and retina_nhlh2_rep2; retina_wt_rep3 & retina_nhlh2_rep3.
Project description:Seven-day-old C57BL/6 mice and IL-19 knockout (KO) mice were subjected to 75% oxygen for 5 days to induce OIR. To explore the mechanism that caused by IL-19 on retinal neovascularization, Gene expressions among retinas of room air control mice, OIR wildtype mice, and OIR IL-19 knockout mice were measured by RNA-sequencing (RNA-seq).
Project description:Nhlh2 is a neuronal helix-loop-helix transcription factor. The goal of this study was to determine the pattern of gene expression in wildtype mouse retina and to compare it to that in the retinas of mice in which the nhlh2 gene had been knocked out.
Project description:Chromatin accessibility was assayed in wildtype or Dppa2 knockout ESC after 26 days of release of the trigger imposed by epigenetic editing. Samples were collected in two clonal knockout and wildtype lines after sorting at FACS of cells which maintained a repressive Esg1-tdTomato (TOMneg) reporter expression after 26 days of DOX washout (release of the trigger).
Project description:Hearts(left ventricles) were harvested from control wildtype and ISG15 knockout mice and wildtype and ISG15 knockout mice 4 weeks after pressure overload induced by transverse aortic constriction. Nano LC-MS/MS analysis was performedon left ventricle tissue following protein extraction, trypsin digestion, peptide desalting, anti K-e-GG enrichment.
Project description:Mice were a mixed background between 129/SvEv and C57BL/6. Rp1 knockout mice (Rp1-/-) and wildtype littermates examined. At P7, P10, P14, P18 and P21, mice were sacrificed and retinas removed. Each RNA sample included a pool of neural retinas from 3-4 mice. Retinas were all collected at 1-2 pm. Keywords: other