Ontology highlight
ABSTRACT:
PROVIDER: PRJNA749049 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR15217764_1.fastq.gz | Fastqsanger.gz | |||
| SRR15217764_2.fastq.gz | Fastqsanger.gz | |||
| SRR15217765_1.fastq.gz | Fastqsanger.gz | |||
| SRR15217765_2.fastq.gz | Fastqsanger.gz | |||
| SRR15217766_1.fastq.gz | Fastqsanger.gz |
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Project description:The long-lived sacred lotus (Nelumbo nucifera) fruit provides a good platform for uncovering aging-related mechanism in extension of seed-life and improving of food quality of economic crops during storage. Here, we investigated the proteome of artificial aging lotus seed to uncover the aging -resistance mechanism. Proteome was performed on the seeds embryo axis treated at 80C for 0, 12 and 24 Hours. We identified 4667 proteins, and found 383 and 37 proteins (folds>1.3, FDR<0.01) were differentially expressed from 0-12h and 12-24h treatment, respectively. This proteome data will provide a better understanding and give further insights on lotus seed aging-resistance.
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Project description:Seeds are comprised of three majors parts of distinct parental origin: the seed coat, embryo, and endosperm. The maternally-derived seed coat is important for nurturing and protecting the seeds during development. By contrast, the embryo and the endosperm are derived from a double fertilization event, where one sperm fertilizes the egg to form the diploid zygote and the other sperm fertilizes the central cell to form the triploid endosperm. Each seed parts undergo distinct developmental programs during seed development. What methylation changes occurring in the different seed parts, if any, remains unknown. To uncover the possible role of DNA methylation in different parts of the seed, we characterized the methylome of three major parts of an early maturation stage seed: seed coat, embryonic cotyledons, and embryonic axis using Illumina sequencing. Illumina sequencing of bisulfite-converted genomic DNA from three parts of an mid-maturation (B1) stage seed: seed coat (B1-SC), embryonic cotyledons (B1-COT), and embryonic axis (B1-AX).
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