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ABSTRACT: white flower RNA-seq in Brassica napus.L

PROVIDER: PRJNA749083 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR15219581.fastq.gz	Fastqsanger.gz
	SRR15219581_1.fastq.gz	Fastqsanger.gz
	SRR15219581_2.fastq.gz	Fastqsanger.gz
	SRR15219582.fastq.gz	Fastqsanger.gz
	SRR15219582_1.fastq.gz	Fastqsanger.gz

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Project description:To cover a comprehensive understanding of the complex regulatory networks controlling cryptochrome 1(CRY1)-mediated photomorphogenic response in Brassica, a genome-wide differential expression analysis was performed for the 5-day-old T3 BnCRY1-OE and wild type seedlings grown under white light (100 M-BM-5mol mM-bM-^@M-^S2sM-bM-^@M-^S1) on 0.8% agar-gelled MS (Murashige and Skoog) medium containing 2% sucrose. Total RNA was extracted and cleaned up with the Qiagen RNeasy Plant Mini Prep Kit as per the manufacturerM-bM-^@M-^Ys instructions (Qiagen). The yield and RNA purity were determined spectrophotometrically. Integrity of the RNA was checked using Agilent Bioanalyzer (Agilent Technologies, USA). 500 ng RNA of each of the sample was processed for microarray analysis according to Agilent protocol. Keywords: Cryptochrome, Brassica, photomorphogenesis, differential expression. Agilent one-color experiment, Organism: B. napus (Brassica) Oligo Microarray, Agilent-022520 B. napus (Brassica) Oligo Microarray 4x44K, Labeling kit: Agilent Quick-Amp labeling Kit (p/n5190-0442)

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Project description:Lily Flower RNA seq

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Production of Red Flower Rapeseed by Ectopic Expression of OvPAP2 Identified in Brassica napus-Orychophragmus violaceus Addition Line by Comparative Transcriptome Analysis

Project description:Oilseed rape is both an important oleaginous crop and agriculture sightseeing crop whereas has relatively scanty flower color. As natural ﬂavonoids, Anthocyanin are responsible for the attractive red, purple, and blue colors of various tissues in higher plants, especially for the ornamental plants flower. One Brassica napus-Orychophragmus violaceus disomic addition line (M4) obtained previously exhibits red petals whichresult from anthocyanin biosynthesis. Transcriptome analysis of M4, B. napus (H3), natural individuals of O. violaceus with purple petals (OvP) and white petals (OvW) revealed that most of structural genes for the anthocyanin synthesis were up-regulated in both M4 and OvP, especially key gene ANS in the last step. Reads assembling and sequence alignment showed that the regulatory DEG PAP2 in M4 was from the transcript of O. violaceus. OvPAP2 was transformed into Arabidopsis thaliana and B. napus driven by the CaMV35S promoter and the rape petal-specific prompter XY355. Transgenic A. thaliana plants showed different levels of purple pigments in most of the organs, including the petals, and transgenic B. napus flowers exhibited restricted accumulation of anthocyanins in stamens when driven by CaMV35S promoter, but generated both red petals and anthers driven by the XY355 promoter. These results provided a platform for expounding the anthocyanin biosynthesis pathway in B. napus petals and give a successful case for flower color modification of the agriculture sightseeing rape.

2017-12-30 | GSE93089 | GEO

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