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Genome-wide localization profile (ChIP-seq) and differential expression profile upon RNAi treatment (RNA-seq) of L(3)mbt and its interactor in ovarian somatic cells[OSCs] in Drosophila

ABSTRACT: Genome-wide localization profile (ChIP-seq) and differential expression profile upon RNAi treatment (RNA-seq) of L(3)mbt and its interactor in ovarian somatic cells[OSCs] in Drosophila

PROVIDER: PRJNA753502 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR15406485_1.fastq.gz	Fastqsanger.gz
	SRR15406485_2.fastq.gz	Fastqsanger.gz
	SRR15406486_1.fastq.gz	Fastqsanger.gz
	SRR15406486_2.fastq.gz	Fastqsanger.gz
	SRR15406487_1.fastq.gz	Fastqsanger.gz

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Genome-wide localization profile (ChIP-seq) and differential expression profile upon RNAi treatment (RNA-seq) of L(3)mbt and its interactor in ovarian somatic cells[OSCs] in Drosophila

Project description:L(3)mbt regulate the transcription of germ-specific genes in somatic cells but the detailed mechanism remains unclear. We performed ChIP-seq of L(3)mbt and a newly identified interactor in OSCs to elucidate the genome-wide localization. Then we performed RNA-seq of OSCs before and after RNAi treatment of L(3)mbt and its interactors and performed differential expression analysis.

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Genome-wide localization profile (ChIP-seq) and differential expression profile upon RNAi treatment (RNA-seq) of L(3)mbt and its interactors in ovarian somatic cells[OSCs] in Drosophila

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Project description:Mutants in the Drosophila gene lethal (3) malignant brain tumor cause malignant growth in the larval brain. This data shows the changes in gene expression profile associated to mutations in l(3)mbt, both in situ in third instar larval brains and in tumors cultured for 1 5 and 10 (T1, T5, T10) rounds of allograft culture We performed genome-wide gene expression profiling of l(3)mbt[E2] and l(3)mbt[ts1] homozygous and transheterozygous larval brains raised at restrictive temperatures (29º C). We obtained three replicates for l(3)mbt[E2] at 17ªC (MBT_E2_17), l(3)mbt[ts1] at 17ºC (MBT_TS1_17), l(3)mbt[E2] at 29ºC (MBT_E2_29) and the transheterozygous at 29ºC (MBT_TS1_ME2), and six replicates l(3)mbt[ts1] at 29ºC (MBT_TS1_29). We also obtained three replicates for l(3)mbt[ts1] tumors at the first (T1), fifth (T5) and tenth (T10) round of allograft culture in adult flies, and six replicates for wild type w[1118] flies (WT).

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