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Transcript profiling of Candida albicans tor1 kinase mutants

ABSTRACT: Transcript profiling of Candida albicans tor1 kinase mutants

PROVIDER: PRJNA755282 | ENA |

REPOSITORIES: ENA

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Transcript profiling of Candida albicans tor1 kinase mutants

Project description:This study analyses the effects of truncation of the gene encoding Tor1 kinase in Candida albicans. The study involves a mutant tetO-TOR1-Del381 which has the N terminal regulator HEAT repeats deleted compared to a full length tetO-TOR1 strain and a heterozygous TOR1/tor1 control. Cell were grown in vitro in YEPD broth at 30˚C. In shut off experiments, doxycycline (30 µg.ml) was added to cultures to shut off the tetO promoter and inhibit Tor1 expression.

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