Project description:This project aims to establish the embryonic anole lung as a model for investigating lung development and more general epithelial morphogenesis by generating a set of single-cell RNA-seq (scRNA-seq) data from late-stage embryonic lungs taken from brown anoles (Anolis sagrei). The anole lung, with its simple architecture, provides a novel tool for investigating signaling in a less complex respiratory system than the murine lung, and this data set would be a large advance in making this system more widely known and accesible among developmental biologists.
Project description:qPCR profiling of miRNAs in mouse fetal lungs was done. Lungs were dissected and RNA was extracted using miRVANA extraction kit (Applied Biosystems).
Project description:Neurons deficient in both GSK-3 alpha and beta isoforms fail to migrate properly and develop abnormal morphology. In exploring mechanisms, we found no change in Wnt transcriptional target genes. Verified decrease in GSK-3B exon 2 RNA was extracted from presumptive somatosensory cortices of E18 GSK-3 alpha -/- beta loxp/loxp (control) and GSK-3:Nex conditional mutant embryos. There were totally 6 samples: 3 control and 3 mutants.
Project description:qPCR profiling of miRNAs in mouse fetal lungs was done. Lungs were dissected and RNA was extracted using miRVANA extraction kit (Applied Biosystems). RNA was isolated from 2 separate lungs (labeled as A or B) for each time point and 2 technical replicates were run for each sample (labeled as 1 or 2).
Project description:The immune system sustains inflammation, and can occasionally inhibit tumor development. Conversely, chronic inflammation positively contributes to the development of cancer. Furthermore, it was revealed that the large number of macrophages and haematopoietic progenitor cells accumulate in response to primary tumors in pre-metastatic lungs. However, the relationship between the inflammation-like state induced by primary tumors in pre-metastatic phase and the migration of tumor cells in metastasis is not well understood. Here we analyzed transcriptional change of the lungs by pre-metastatic stimuli. Keywords: Gene expression analysis of pre-metastatic lungs