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Genome-wide expression analysis of Arabidopsis Col-0 plant and clf mutant plant under high humidity [RNA-seq]

ABSTRACT: Genome-wide expression analysis of Arabidopsis Col-0 plant and clf mutant plant under high humidity [RNA-seq]

PROVIDER: PRJNA761292 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR15736802_1.fastq.gz	Fastqsanger.gz
	SRR15736802_2.fastq.gz	Fastqsanger.gz
	SRR15736803_1.fastq.gz	Fastqsanger.gz
	SRR15736803_2.fastq.gz	Fastqsanger.gz
	SRR15736804_1.fastq.gz	Fastqsanger.gz

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Genome-wide expression analysis of Arabidopsis Col-0 plant and clf mutant plant under high humidity [RNA-seq]

Project description:Purpose: The goal of this study is to demonstrate the global expression profile of Arabidopsis wild type and clf mutant plants in response to high air humidity Methods: Four-week-old Arabidopsis plant leaves were treated with ~95% relative humidity for 0.5 hour (0h as control) Results and Conclusions: This study showed that many genes including ABA-responsive genes and stomotal movement-related genes were differentially expressed in the clf mutant plant.

2024-09-06 | GSE183558 | GEO

Genome-wide histone 3 lysine 27 trimethylation patterns in wild type and CLF-deficient Arabidopsis plants after high humidity treatment [ChIP-seq]

Project description:We report the H3K27 trimethylation profiles in Arabidospsis wild type and clf mutant plants before and after high humidity treatment. We find that many genes display a different H3-lysine 27 trimethylation level in the clf mutant plant compared to Col-0 plant. In addition, high humidity also influences H3K27 trimethylation profiles in both genotypes.

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Genome-wide expression analysis of Arabidopsis Col-0 plants under different air humidity

Project description:The goal of this study is to demonstrate the global expression profile of Arabidopsis Col-0 wild type plant leaves in response to low, moderate and high humidity.

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Genome-wide expression analysis of Arabidopsis Col-0 plants under BTH treatment and high or low humidity

Project description:The goal of this study is to demonstrate the global expression profile of four-week-old Arabidopsis Col-0 wild type plant leaves in response to high or low air humidity, under water or BTH (SA analog) treatment.

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Genome-wide expression analysis of Arabidopsis Col-0 plants under different air humidity

Project description:Genome-wide expression analysis of Arabidopsis Col-0 plants under different air humidity

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Genome-wide analysis reveals CLF-regulated transcripts in Arabidopsis

Project description:CURLY LEAF (CLF), the major histone methyltransferase of Polycomb Repressive Complex 2 (PRC2), modifies trimethylation of histone H3 lysine 27 (H3K27me3) and mediates dynamical chromatin repression in Arabidopsis. Here we profiled Arabidopsis transcriptomes obtained from roots, leaves, flowers and siliques of Col-0 and clf-28 plants using RNA-seq. Our analysis uncovered 3835 transcription units were up-regulated in clf-28. Compared with H3K27me3 ChIP-CHIP data, we found at least 42% of them were associated with H3K27me3.

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Identification of CLF-regulated transcripts using strand-specific RNA-sequencing in Arabidopsis

Project description:CURLY LEAF (CLF), the major histone methyltransferase of Polycomb Repressive Complex 2 (PRC2), modifies trimethylation of histone H3 lysine 27 (H3K27me3) and mediates dynamical chromatin repression in Arabidopsis. Here we used strand specific RNA-sequencing to profile Arabidopsis transcriptomes obtained from roots, shoots, flowers and siliques of Col-0 and clf-28 plants. Our analysis identified a large number of CLF-regulatedd transcripts in Arabidopsis.

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Expression data from Col, brm-1, clf-29 and brm-1 clf-29 14-d-old seedlings

Project description:To understand how actions of the chromatin-remodeler BRAHMA (BRM) and Polycomb Group (PcG) proteins are coordinated during plant development, we performed a genome-wide profiling of trimethylated histone H3 lysine 27 (H3K27me3) in brm mutant seedlings by chromatin immunoprecipitation followed by next generation sequencing (ChIP-seq). Increased H3K27me3 deposition at several hundred genes was observed in brm mutants and this increase was partially supressed upon removal of the CURLING LEAF (CLF) H3K27me3 methyltransferase. ChIP experiments demonstrated that BRM directly binds to a subset of genes and prevents the inappropriate association of PcG proteins at some of the loci. Together, these results indicate a crucial role of BRM in restricting the inappropriate activity of PcG protein complexes during plant development.

2015-03-09 | GSE53621 | GEO

RNA-seq of Arabidopsis thalianal Col-0 wild type and clf-29 lhp1-4 mutant root

Project description:RNA-seq was performed in order to identify qualitative variation of transcripts in clf-29 lhp1-4 compared to WT, including SNPS, indels, splicing fidelity

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Genome-wide analysis reveals CLF-regulated transcripts in Arabidopsis

Project description:CURLY LEAF (CLF), the major histone methyltransferase of Polycomb Repressive Complex 2 (PRC2), modifies trimethylation of histone H3 lysine 27 (H3K27me3) and mediates dynamical chromatin repression in Arabidopsis. Here we profiled Arabidopsis transcriptomes obtained from roots, leaves, flowers and siliques of Col-0 (As described under GEO ID: GSE38612) and clf-28 plants using RNA-seq. Our analysis uncovered 3835 transcription units were up-regulated in clf-28. Compared with ChIP-CHIP data, we found at least 42% of them were associated with H3K27me3. Transcriptom profiling in roots, leaves, flowers and siliques of clf-28 plants.

2015-02-01 | E-GEOD-43983 | biostudies-arrayexpress

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