Ontology highlight
ABSTRACT:
PROVIDER: PRJNA764457 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:The effects of phoenixin-14 on the hypothalamic-pituitary-gonadal (HPG) axis and spawning in green-spotted puffer (Dichotomyctere nigroviridis)
2022-03-01 | GSE183029 | GEO
Project description:Pregnancy is a unique and critical period in a female’s life during which the whole organism is mobilized and focused on ensuring the successful survival and development of the embryo. The main regulatory system responsible for the reproductive functions during both the oestrous cycle and pregnancy is the hypothalamic-pituitary-gonadal (HPG) axis. Herein we verified hypothesis assuming the modulatory effect of visfatin on the anterior pituitary transcriptome during the peri-implantation period. We analyzed samples obtained from 5 individuals (n=5). After the enzymatic tissue digestion with the use collagenase V and pancreatin cells were divided for two groups: controls [without treatment] and visfatin (100 ng/mL) treated ones. In vitro cell cultures were conducted for 24 hours.
2024-09-30 | E-MTAB-14347 | biostudies-arrayexpress
Project description:Ultraviolet (UV) light affects endocrinological and behavioral aspects of human sexuality via an unknown mechanism. Using a unique male-female comparative approach, we discovered that the sexual behavioral and hormonal features enhanced by UVB are mediated by the skin. In mice, UV exposure increases hypothalamus-pituitary-gonadal axis hormone levels, resulting in enhanced ovary size, extended estrus days, and anti-Mullerian hormone (AMH) expression. It likewise enhances the sexual responsiveness and attractiveness of females and male-female interactions of both males and females. Conditional knockout of p53 specifically in skin keratinocytes abolished UV’s effects. In humans, UV exposure enhanced romantic passion in both genders increased testosterone levels in men. Our data, revealing that UVB triggers a skin-brain-gonadal axis through skin p53 activation, offers therapeutic opportunities for sex-steroid-related dysfunctions. We speculate that during human furless skin evolution, the skin became the front-line regulator of the response to UVB.
2022-02-17 | PXD025973 | Pride
Project description:Maternal stress during late pregnancy can lead to intrauterine hyperthermia affecting fetal hypothalamic-pituitary-adrenal axis development and function. This study aimed to characterize the impact of in utero heat stress on the postnatal offspring's adrenal gland transcriptome.
2023-06-21 | GSE235160 | GEO
Project description:Emerging studies have reported a significant correlation between obesity and a decline in sperm quality among males, however, the underlying mechanism remains elusive. In this study, we observed that male mice with diet-induced obesity (DIO) exhibited functional alteration in the hypothalamic-pituitary-gonadal (HPG) axis, as evidenced by disruption of luteinizing hormone (LH) pulse release. This alteration was attributed to the activation of nuclear factor kappa B subunit (NF-κB) signaling in the hypothalamus, which subsequently led to a decline in sperm quality. The application of RNA-seq analysis in the hypothalamic arcuate nucleus (ARC) of DIO male mice has revealed a signaling network implicating protein phosphatase 2 catalytic subunit alpha (Ppp2ca), which is involved in the disruption of LH pulse secretion. Activation of NF-κB signaling increased expression, which decreased the Kiss1 expression through inhibition of Akt kinase (AKT) and cAMP responsive element binding protein 1 (CREB1) activities. Overexpression of the Ppp2ca gene in the hypothalamic ARC resulted in disrupted LH pulse secretion patterns and reduced sperm quality. Collectively, our findings provide novel insights into the molecular mechanisms underlying the decline in sperm quality observed in male DIO mice.
2025-03-05 | GSE284408 | GEO
Project description:Hypothalamic -pituitary axis changes related to growth rate in Kołudzka White geese
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Project description:Enhanced nutrition during the early calfhood period has been shown to hasten the onset of puberty in heifer calves. The hypothalamic-pituitary-ovarian biochemical signalling axis regulates reproductive development in heifers, with the dynamics of gonadotropin pulsatility within the anterior pituitary gland, in particular, central to final sexual maturity. However, the precise molecular mechanisms regulating the influence of metabolic status on this signalling axis in heifer calves is yet to be fully elucidated. The objective of this study was to determine the effect of an enhanced plane of nutrition during early life, up to 21 weeks of age, on the anterior pituitary gland proteome of heifer calves. Heifer calves were offered either a high or moderate plane of nutrition. Pathway analysis of differentially abundant proteins highlighted an effect on both growth hormone and GnRH signalling pathways, as well as an effect towards reproductive system development and function
2024-07-04 | PXD042011 | Pride
Project description:Kisspeptin-expressing neurons in the rostral periventricular region of the third ventricle (RP3V) play an essential role in female reproduction. However, adult male mice were reported to have very few Kisspeptin-expressing neurons in the RP3V compared to females. This led to the hypothesis that Kiss1 RP3V neurons are responsible for the ability of females, but not males, to generate a surge of LH, triggering ovulation and steroid synthesis in the female. Using mouse genetics and cell type-specific gene expression analysis, we show that male mice harbor almost as many Kiss1 RP3V neurons as the female and that gene expression in these neurons is very similar. Specific activation of male Kiss1 RP3V neurons expressing viral-encoded hM3Dq caused a surge in serum testosterone levels. These results demonstrate that Kiss1 RP3V neurons are present in the adult male and fully capable of regulating the hypothalamic/pituitary/gonadal axis. We suggest that these neurons may continue to play a role in reproductive behavior in adult male mice.
2021-06-01 | GSE165015 | GEO
Project description:Recent genome-wide association studies (GWAS) identified Dusp8, a dual-specificity phosphatase targeting MAP kinases, as type 2 diabetes risk gene. Here, we unravel Dusp8 as gatekeeper in the hypothalamic control of glucose homeostasis in mice and humans. Male but not female Dusp8 loss-of-function mice, either with global or CRH neuron-specific deletion, had impaired systemic glucose tolerance and insulin sensitivity when exposed to high-fat diet (HFD). Mechanistically, we found impaired hypothalamic–pituitary–adrenal (HPA) axis feedback, blunted sympathetic responsiveness, and chronically elevated corticosterone levels driven by hypothalamic hyperactivation of Jnk signaling. Accordingly, global Jnk1 ablation, AAV-mediated Dusp8 overexpression in the mediobasal hypothalamus, or metyrapone-induced chemical adrenalectomy rescued the impaired glucose homeostasis of male Dusp8 KO mice, respectively. This sex-specific and rheostatic role of murine Dusp8 in governing hypothalamic Jnk signaling, insulin sensitivity and systemic glucose tolerance was consistent with fMRI data in human volunteers that revealed an association of the DUSP8 rs2334499 risk variant with hypothalamic insulin resistance in men. In summary, our findings suggest GWAS-identified gene Dusp8 as novel hypothalamic factor that plays a functional role in the etiology of type 2 diabetes.
2020-08-17 | PXD019451 | Pride