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Human resting natural killer (NK) cells, activated NK cells and their secreted small EVs have a specific miRNA repertoire with a distinct pattern of non-templated post-transcriptional modifications

ABSTRACT: Human resting natural killer (NK) cells, activated NK cells and their secreted small EVs have a specific miRNA repertoire with a distinct pattern of non-templated post-transcriptional modifications

PROVIDER: PRJNA767862 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR16151900.fastq.gz	Fastqsanger.gz
	SRR16151901.fastq.gz	Fastqsanger.gz
	SRR16151902.fastq.gz	Fastqsanger.gz
	SRR16151903.fastq.gz	Fastqsanger.gz
	SRR16151904.fastq.gz	Fastqsanger.gz

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Human resting natural killer (NK) cells, activated NK cells and their secreted small EVs have a specific miRNA repertoire with a distinct pattern of non-templated post-transcriptional modifications

Project description:NGS-based assesement of miRNA expression and post-transcriptional modification kinetics in human primary resting and activated natural killer (NK) cells and their released small EVs

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Project description:In this study we have compared the proteomic profile of extracellular vesicles (EVs) prepared from primary, human NK cells or the human NK cell lines NK-92 and KHYG-1 cultured for 48hrs in serum-free conditions. EVs were harvested from cells either under resting conditions (culture in IL-15) or upon activation (combination of IL-12, IL-15, and IL-18). In addition, primary NK cells were activated in the presence of anti-CD16-coated beads, and EVs harvested after 48hrs. The aim was to compare their ability to target and kill a variety of tumor cell line-derived spheroids

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Project description:Resting NK cells are notoriously difficult to transduce, especially using the VSVG lentivirus pseudotype. When NK cells are expanded and activated using the NKAES system, there is a modest improvement of transduction rates compared to their resting NK counterparts. Still, the transduction efficiency remains poor for robust development of NK cell-based cancer immunotherapy. We envisaged to have a genome-wide expression profile of resting and activated NK (NKAES) cells in order to improve currrent transduction protocols .

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Project description:Genome-wide transcriptional profiling was used to identify gene expression signatures and pathways in resting and IL2 activated NK cell isolated from peripheral blood of healthy donors Keywords: Time series_NK cells gene profile

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Microarray analysis of resting Nkrp1g+ NK cells versus resting Nkrp1g- NK cells from spleens of female BALB/c mice.

Project description:Natural Killer Gene Complex (NKC)-encoded immunomodulatory C-type lectin-like receptors (CTLR) include members of the NKRP1 and CLEC2 gene families, which constitute genetically linked receptor-ligand pairs and are thought to allow for NK cell-mediated immunosurveillance of stressed or infected tissues. The mouse CTLR Nkrp1g was previously shown to form several receptor-ligands pairs with the CLEC2 proteins Clr-d, Clr-f, and Clr-g, respectively. Recently, we demonstrated a gut-restricted expression of Clr-f on intestinal epithelial cells that is spatially matched by Nkrp1g on subsets of intraepithelial lymphocytes (Leibelt et al., 2015). We now investigated expression and ligand interaction of Nkrp1g in the splenic compartment, and found an exclusive expression of Nkrp1g on a small subset of NK cells that upregulates Nkrp1g after cytokine exposure. To further characterize NKrp1g+ NK cells, we performed microarray analysis of resting Nkrp1g+ versus Nkrp1g- splenic NK cells that were purified by FACsorting. Total RNA isolation was followed by an Affymetrix microarray analysis.

2017-11-21 | E-MTAB-6223 | biostudies-arrayexpress

A comparison of the transcriptional profiles of resting NK cells and ex vivo expanded NK cells

Project description:NK cells are cytotoxic lymphocytes that play an important role in the innate immune response. The immune response of NK cells was shown under activated conditions. In order to identify such patterns, we performed RNA sequencing to confirm the gene expression profiles in NK cells expanded ex vivo compared to that of resting NK cells.

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Effect of resting or activated human regulatory T cells (Tregs) on human natural killer (NK) cell differentiation in vitro.

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Resting natural killer cell homeostasis relies on tryptophan/NAD+ metabolism and HIF-1α

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Project description:To investigate miRNA expressions of miRNA upon activation. In vitro-differentiated human NK cells were freshly incubated in the presence of IL15 after 24h deprivation of IL-15. The cells were harvested at the times (0h, resting-Sample 1 and 2; 6h, activated-Sample 3 and 4) and analyzed by microarray.

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