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Comprehensive DNA methylation analysis of human neuroblastoma cells treated with haloperidol and risperidone

ABSTRACT: Comprehensive DNA methylation analysis of human neuroblastoma cells treated with haloperidol and risperidone

PROVIDER: PRJNA771630 | ENA |

REPOSITORIES: ENA

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Comprehensive DNA methylation analysis of human neuroblastoma cells treated with haloperidol and risperidone

Project description:This work reveals the effect of antipsychotics on DNA methylation using the cell model. After the treatment of antipsychotics, haloperidol and risperidone, DNA methylation profiles obtained by Illumina HumanMethylation450 beadchip were examined.

2021-11-02 | GSE185973 | GEO

Comprehensive DNA Methylation Analysis of Human Neuroblastoma Cells Treated With Haloperidol and Risperidone.

Project description:Accumulating evidence suggests that the epigenetic alterations induced by antipsychotics contribute to the therapeutic efficacy. However, global and site-specific epigenetic changes by antipsychotics and those shared by different classes of antipsychotics remain poorly understood. We conducted a comprehensive DNA methylation analysis of human neuroblastoma cells cultured with antipsychotics. The cells were cultured with low and high concentrations of haloperidol or risperidone for 8 days. DNA methylation assay was performed with the Illumina HumanMethylation450 BeadChip. We found that both haloperidol and risperidone tended to cause hypermethylation changes and showed similar DNA methylation changes closely related to neuronal functions. A total of 294 differentially methylated probes (DMPs), including 197 hypermethylated and 97 hypomethylated DMPs, were identified with both haloperidol and risperidone treatment. Gene ontology analysis of the hypermethylated probe-associated genes showed enrichment of genes related to the regulation of neurotransmitter receptor activity and lipoprotein lipase activity. Pathway analysis identified that among the DMP-associated genes, SHANK1 and SHANK2 were the major genes in the neuropsychiatric disorder-related pathways. Our data would be valuable for understanding the mechanisms of action of antipsychotics from an epigenetic viewpoint.

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Microarray profiling of rats treated for 21 days with haloperidol (0.25 mg/kg/d), risperidone (5 mg/kg/d) or vehicle

Project description:SD rats were dosed with gold standard antipsychotic therapies, haloperidol or risperidone, at doses aimed at achieving therapeutically relevant drug exposures. Tissues were collected after 21 days of delivery to examine the impact of this treatment on gene expression in frontal cortex, hippocampus and striatum.

2015-03-16 | GSE66275 | GEO

Expression data SK-N-SH cells treated with risperidone

Project description:We performed a pharmacotranscriptomic analysis of a human neuroblastoma cell line (SK-N-SH) exposed to risperidone to identify molecular mechanisms involved in the cellular response to risperidone and thus identify candidate genes for pharmacogenetic studies. This study is a part of a convergent functional genomic approach that plans to integrate the data presented here with: data from gene expression analysis of experimental animal brain under treatment with risperidone; and data from gene expression analysis of peripheral blood from first psychotic patients treated with risperidone.

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Expression data SK-N-SH cells treated with risperidone

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C6 glioblastoma microRNA regulation induced by haloperidol, risperidone and clozapine

Project description:C6 glioblastoma microRNA regulation induced by haloperidol, risperidone and clozapine

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Transcription profiling of mouse brain treated with clozapine and haloperidol

Project description:This SuperSeries is composed of the following subset Series:; GSE6467: Twelve weeks expression data of the antipsychotics Clozapine and Haloperidol in the mouse brain (Affymetrix, GCRMA). GSE6511: Four weeks expression data of the antipsychotics Clozapine and Haloperidol in the mouse brain (Affymetrix, GCRMA). Experiment Overall Design: Refer to individual Series

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Evaluation and mechanism study of cardiotoxicity caused by antipsychotics using human-induced pluripotent stem cell-derived cardiomyocytes

Project description:In this study, a comprehensive evaluation model for studying the cardiac toxicity of antipsychotic drugs was established by using iPSC-CMs. Six antipsychotic drugs, including aripiprazole, risperidone, quetiapine, haloperidol, clozapine, and olanzapine, all induced concentration-dependent QT prolongation in iPSC-CMs upon acute administration, and high concentrations of these drugs caused disorganized sarcomere arrangement in iPSC-CMs.

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Comprehensive DNA methylation analysis of human neuroblastoma cells treated with blonanserin and perospirone

Project description:Comprehensive DNA methylation analysis of human neuroblastoma cells treated with blonanserin and perospirone

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