Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA sequence of mRNA after silencing of HGDILnc1 compared to controls.

ABSTRACT: RNA sequence of mRNA after silencing of HGDILnc1 compared to controls.

PROVIDER: PRJNA773971 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR16550191_1.fastq.gz	Fastqsanger.gz
	SRR16550191_2.fastq.gz	Fastqsanger.gz
	SRR16550192_1.fastq.gz	Fastqsanger.gz
	SRR16550192_2.fastq.gz	Fastqsanger.gz
	SRR16550193_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 16

Similar Datasets

RNA microarray of mRNA after after hypoxia or glucose deprivition compared to controls.

Project description:RNA microarray of mRNA after after hypoxia or glucose deprivition compared to controls.

| PRJNA773970 | ENA

RNA sequence of mRNA after silencing of HGDILnc1 compared to controls.

Project description:We report a total of 692 downregulated genes and 916 upregulated genes (2-fold) were detected after knockdown of HGDILnc1 in HUVEC cells

2024-03-20 | GSE186473 | GEO

RNA-Seq analysis of SRSF4 KO hearts compared to controls.

Project description:RNA-Seq analysis of SRSF4 KO hearts compared to controls.

| PRJNA508380 | ENA

Coding sequence targeting by MicC RNA reveals bacterial mRNA silencing downstream of translational initiation

Project description:It is well established that small noncoding RNAs (sRNAs) of bacteria recognize the 5’ regions of target mRNAs, generally at the ribosome binding site. Until now, the translated coding sequence (CDS) of mRNA appeared to be refractory to sRNA interactions. We have discovered that Salmonella MicC RNA silences ompD mRNA by targeting the CDS, at codons 23-26. Analyses of MicC-ompD RNA complexes in vitro, and of chimeric sRNAs and ompD reporter fusions in vivo, show that interactions are confined to the CDS, and that a ≤12 bp RNA duplex involving the conserved 5’ end of MicC is essential and sufficient for target repression. MicC cannot act as a translational repressor at this downstream position being unable to inhibit 30S or 70S ribosome activity on the ompD mRNA. Instead, MicC accelerates RNase E-dependent ompD mRNA decay. The degradosome contributes to target destabilization, and facilitates the efficient degradation of processed ompD mRNA. Our data show that bacterial gene silencing by sRNAs can take place downstream of the translational initiation site by triggering irreversible mRNA decay. This ability of sRNAs allows targets in the CDS to be silenced without interference from the strong RNA helicase activity of elongating ribosomes.

2009-07-16 | GSE16540 | GEO

Effects of circHIPK3 silencing on mRNA expression

Project description:Effects of circHIPK3 silencing on mRNA expression

| PRJNA414627 | ENA

Coding sequence targeting by MicC RNA reveals bacterial mRNA silencing downstream of translational initiation

Project description:It is well established that small noncoding RNAs (sRNAs) of bacteria recognize the 5â?? regions of target mRNAs, generally at the ribosome binding site. Until now, the translated coding sequence (CDS) of mRNA appeared to be refractory to sRNA interactions. We have discovered that Salmonella MicC RNA silences ompD mRNA by targeting the CDS, at codons 23-26. Analyses of MicC-ompD RNA complexes in vitro, and of chimeric sRNAs and ompD reporter fusions in vivo, show that interactions are confined to the CDS, and that a â?¤12 bp RNA duplex involving the conserved 5â?? end of MicC is essential and sufficient for target repression. MicC cannot act as a translational repressor at this downstream position being unable to inhibit 30S or 70S ribosome activity on the ompD mRNA. Instead, MicC accelerates RNase E-dependent ompD mRNA decay. The degradosome contributes to target destabilization, and facilitates the efficient degradation of processed ompD mRNA. Our data show that bacterial gene silencing by sRNAs can take place downstream of the translational initiation site by triggering irreversible mRNA decay. This ability of sRNAs allows targets in the CDS to be silenced without interference from the strong RNA helicase activity of elongating ribosomes. To determine the targets of the small regulatory RNA MicC in S. Typhimurium, we looked at the effect of a short pulse of MicC over-expression on the Salmonella transcriptome. To achieve over-expression, the micC gene was cloned in the pBAD plasmid and induced with 0.2% L-arabinose for 10 min. We then extracted the total RNA for transcriptional profiling. A strain carrying the pBAD plasmid w/o insert was used as negative control. 3 biological replicates were performed. This sRNA target identification strategy has been described in Papenfort et al; Molecular Microbiology (2006) 62(6), 1674â??1688.

2010-05-16 | E-GEOD-16540 | biostudies-arrayexpress

GnRH Agonist-Induced Ovarian Suppression and Ovarian Steroids in PMDD and Controls

Project description:<p>In this study, lymphoblastoid cell line cultures (LCLs) from women with premenstrual dysphoric disorder (PMDD) and asymptomatic controls were compared via whole-transcriptome sequencing (RNA-seq) during untreated (ovarian steroid-free) conditions and after estradiol and progesterone treatment. In untreated LCLs, pathway analysis of the LCL transcriptome revealed, among others, over-expression of ESC/E(Z) complex genes (an ovarian steroid-regulated gene silencing complex) with significant overexpression of MTF2, PHF19, and SIRT1 (P<0.05) in cells from women with PMDD compared with controls. Finally, mRNA expression of several ESC/E(Z) complex genes were increased by progesterone in controls only, and decreased by estradiol in PMDD LCLs. </p>

| phs001344 | dbGaP

RNA sequencing of AHNAK2 knockdown adenocarcinoma cells compared with the scramble controls

Project description:RNA sequencing of AHNAK2 knockdown adenocarcinoma cells compared with the scramble controls

| PRJNA746132 | ENA

Zc3h10 controls pre-mRNA profile in preadipocytes

Project description:Zc3h10 controls pre-mRNA profile in preadipocytes

| PRJNA622579 | ENA

FOXO-inhibited iPSC-derived cardiomyocytes compared against DMSO vehicle controls.

Project description:FOXO-inhibited iPSC-derived cardiomyocytes compared against DMSO vehicle controls.

| PRJNA1061534 | ENA