Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Injury response of human lens epithelial cells ex-vivo

ABSTRACT: Injury response of human lens epithelial cells ex-vivo

PROVIDER: PRJNA775637 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Injury response of human lens epithelial cells ex-vivo

Project description:This study investigates the response of human lens epithelial cells to mechanical injury. Human geriatric lenses obtained from cadaver eyes from donated to an eye bank for research were subject to in-vitro capsulotomy mimicking the injury sustained during cataract surgery. The anterior capsule was dissected using a curvilinear capsulorhexis technique, and central lens epithelial cells attached to the patch of anterior capsule (Rhexis) were immediately stabilized in RNAlater. The fiber cells were then removed, and the cortical fibers were immediately stabilized in RNA later. The remaining equatorial lens epithelial cells attached to the capsular bag from one eye were stabilized in RNA later immediately while the equatorial lens epithelial cells from the other eye were cultured for 24 hours then stabilized in RNAlater.

2024-10-27 | GSE186716 | GEO

The six-hour injury response in lens epithelial cells

Project description:This trancriptome analysis reports genes that are differentially expressed in wildtype (WT) lens epithelial cells (LEC) between 6 hours and zero hours after injury.

2022-11-16 | GSE206563 | GEO

The six-hour injury response in lens epithelial cells

Project description:The six-hour injury response in lens epithelial cells

| PRJNA851377 | ENA

Epidermal gene expression in wound healing and after ex vivo injury

Project description:Human in vivo skin wound: Non-wounded skin was obtained by taking punch biopsies from three healthy donors (donor 1,2 and 3). The samples were termed 'skin day 0 in vivo wound'. Skin wound samples were retrieved by making new punch biopsies from the edge of the original biopsies after four days. These samples were termed 'skin day 4 in vivo wound'. As much dermal tissue as possible was removed by dissection to make sure mainly epidermis was present in the samples. The samples were washed in NaCl to possible remove infiltrating inflammatory cells before RNA isolation. Ex vivo skin wounds: Skin was obtained from three healthy donors following reduction surgery (donor 1, 2, and 3). As much dermal tissue as possible was removed dissection. These samples were termed 'skin day 0 ex vivo wound'. Skin was sliced into 1x10 mm slices and incubated in keratinocyte medium for four days with either 1:1000 fold dilution of DMSO or 10 micromolar AG-1478 (dissolved in DMSO). Again as much dermal tissue was removed by dissection as possible before RNA was isolated. These samples were termed 'skin day 4 ex vivo wound' and 'skin day 4 AG-1478 ex vivo wound'. By comparing the gene expression day 4 in ex vivo wound with in vivo wounds it was possible to see which part of the gene expression in wounded skin that was due to the epidermal reaction to injury and how much was due to stimuli from infiltrating inflammatory cells absent in the ex vivo skin wounds. By comparing the data from ex vivo skin wounds day 4 with and without the EGFR-inhibitor AG-1478, it was possible to look at the importance of the EGF-receptor of EGFR for the gene expression in ex vivo wounded skin.

2011-09-13 | E-MEXP-3305 | biostudies-arrayexpress

Lens epithelial cells transcriptome analysis

Project description:We performed RNAseq of passage 15 and passage 42 lens epithelial cell (FHL124), a lens epithelial cellular aging model.

2022-07-15 | GSE184422 | GEO

Lens Epithelial Cells and Lens Cortical Fiber Cells

Project description:Total RNA was isolated from three separate populations of human lens epithelial cells and three matching populations of lens cortical fiber cells. All samples were analyzed on separate microarrays. Keywords: repeat sample

2005-02-15 | GSE2256 | GEO

Changes in gene expression time 0 vs. 1hr post-cataract sugery wounding in the lens

Project description:Early changes in the transcriptome associated with lens wounding in an ex vivo post-cataract surgery chicken model. Here we report the changes in the transcriptome that occur 1hr vs. time 0 post-cataract surgery wounding. Our data provide a molecular framework for understanding the early gene changes associated with the injury response of the lens.

2023-11-04 | GSE239368 | GEO

Transcription profiling of human lens epithelial cells and lens cortical fiber cells

2007-08-25 | E-GEOD-2256 | biostudies-arrayexpress

Expression data from rat lens epithelial cells after extracapsular lens extraction (ECLE)

Project description:The epithelial-mesenchymal transition (EMT) of lens epithelial cells (LECs) has been proposed as a major cause of posterior capsule opacification (PCO) after cataract surgery. Molecular mechanism of PCO progression is still unclear. Using a microarray-based approach, herein we studied the changes in gene expression pattern during rat PCO formation in vivo as a model.

2020-09-30 | GSE152593 | GEO

Lens epithelial cells transcriptome analysis

Project description:Lens epithelial cells transcriptome analysis

| PRJNA764592 | ENA