Project description:To investigate the effect of recombinant CXCL12 on patient-derived glioblastoma stem cells, we treated GSCs with recombinant CXCL12 in cell culture media
Project description:Background: Mental Retardation occurs with the prevalence of 2%-3% of general population. Molecular karyotyping by 1-Mb resolution microarray revealed that pathological genomic imbalances were found in 14%-20% of MR. Aim: The aim of this study is to find the submicroscopic rearrangements in patient with MR using the custom BAC microarray (probe spacing at 0.75 Mb throughout the human genome). Conclusion: We identified approximate 2.0-Mb deletion on 9q33.3-q34.11 in a female with MR (Patient 1). Keywords: array CGH
Project description:Patient-derived cancer cells (PDCs) were established by three-dimensional (3D) spheroid culture from testicular germ cell tumor (GCT) specimens. Microarray expression analysis revealed that cancer stem-like cell-related genes were upregulated in 3D culture condition compared with two-dimensional (2D) culture condition.
Project description:We report a new method for culturing patient breast tumor cells in vitro, for subsequent transplantation in vivo for experimental metastasis assays. RNA sequencing shows that tumor spheres formed in culture maintain the fundamental properties of native tumor cells.