Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Bulk RNAseq of Mouse Splenic Dendritic Cell Subsets

ABSTRACT: Bulk RNAseq of Mouse Splenic Dendritic Cell Subsets

PROVIDER: PRJNA780959 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR16960900_1.fastq.gz	Fastqsanger.gz
	SRR16960900_2.fastq.gz	Fastqsanger.gz
	SRR16960901_1.fastq.gz	Fastqsanger.gz
	SRR16960901_2.fastq.gz	Fastqsanger.gz
	SRR16960902_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 18

Similar Datasets

Bulk RNAseq of Mouse Splenic Dendritic Cell Subsets

Project description:Dendritic cells (DCs) are comprised of a heterogenous population. Here we characterize the transcriptomic profiles of mouse splenic DC subsets at steady state to highlight key differences between subsets.

2022-02-01 | GSE188992 | GEO

Splenic Dendritic Cell Subsets

Project description:The functional relationships and properties of different sub-types of dendritic cells (DC) remain largely undefined. We used a global gene profiling approach to determine gene expression patterns among murine splenic CD11c high DC subsets in an effort to better characterise these cells. Keywords: equivalent probe

2003-06-09 | GSE339 | GEO

Expression data from subsets of mouse spleen dendritic cells

Project description:Mouse splenic dendritic cells are divided into different subsets based on their phynotype. CD8Î±+ and CD8Î±- dendritic cells play different roles against pathogens. Cross-presentation is essential for immune defense against viruses, tumors and intracellular bacteria and CD8Î±+ DCs are more potent in cross-presentation compared to CD8Î±- DCs. We used microarrays to detail the global gene expression to analyze the underlying mechanism in CD8Î±+ dendritic cells cross-presentation Splenic CD8Î±+ and CD8Î±- dendritic cells were sorted by flow-cytometer for RNA extraction and hybridization on Affymetrix microarrays. Thirty C57BL/6J mice were sacrified for the splenic cells, splenic DCs were enriched by negtive selection, then CD8Î±+ and CD8Î±- dendritic cells were seprated based on CD11c and CD8Î± markers.

2016-07-27 | E-GEOD-64124 | biostudies-arrayexpress

Bulk RNAseq for B1a B cells of WT and IRF4 T95R mutant mice

Project description:Bulk RNAseq of splenic B1a B cells from wild-type and IRF4 T95R heterozygous mice.

2023-06-01 | E-MTAB-11262 | biostudies-arrayexpress

Gene expression profiles of mouse splenic macrophages and CD8+ and CD8- conventional dendritic cells

Project description:The close functional relationship between macrophages and dendritic cells has long been recognised. Here, we have examined the gene expression profiles of splenic macrophages and the splenic resident dendritic cell subsets, and demostrate that macrophages and DC show different gene expression profiles. Further, we show that the DC subsets are closer to one another in gene expression profile than they are to macrophages. We here identify a list of differentially expressed genes between the DC subsets, and between DC and macrophages Splenic macrophages, CD8+ and CD8- cDC were analyzed

2011-06-17 | E-GEOD-30022 | biostudies-arrayexpress

Proteomics of human dendritic cell subsets

Project description:We exploited label-free quantitative mass spectrometry to compare primary human blood Dendritic cells (DCs) subsets protein expression to identify new markers. Subsets distinguished are: Plasmacytoid DCs (pDC) and BDCA3+ and CD1c+ myeloid DCs and CD16+ monocytes. The dendritic cells were analyzed by LC-MS/MS and processed by MaxQuant for identification and LFQ quantification.

2016-09-27 | PXD004678 | Pride

Gene expression profiles of mouse splenic macrophages and CD8+ and CD8- conventional dendritic cells

2011-06-17 | GSE30022 | GEO

Expression data from subsets of mouse spleen dendritic cells

Project description:Mouse splenic dendritic cells are divided into different subsets based on their phynotype. CD8α+ and CD8α- dendritic cells play different roles against pathogens. Cross-presentation is essential for immune defense against viruses, tumors and intracellular bacteria and CD8α+ DCs are more potent in cross-presentation compared to CD8α- DCs. We used microarrays to detail the global gene expression to analyze the underlying mechanism in CD8α+ dendritic cells cross-presentation

2016-07-27 | GSE64124 | GEO

Fibroblastic reticular cells in the spleen - bulk RNAseq

Project description:Splenic white pulp (WP) structures are underpinned by fibroblastic stromal cells (FSCs) to facilitate splenic compartmentalization and execute efficient immune responses. Although distinct WP FSCs exhibit various molecular traits, the origin and the hierarchical differentiation of different cell subsets are not characterized. Here we showed, the organization of splenic WP and the differentiation of WP FSCs were governed by lymphotoxin beta receptor (LTβR) signaling pathway. Cell fate mapping analysis revealed that different WP fibroblastic stromal cells descend from a common perivascular LTβR-sensitive mesenchymal lymphoid organizer cells (mLTo) at prenatal stage. Moreover, embryonic mLTo cells required LTβR signaling to give rise to different WP stromal cell subsets, while the proliferation of these cells was devoid of LTβR signaling but followed the development of WP during ontogeny. Moreover, cell fate mapping from different time point indicated a consecutive commitment of mLTo cells initiated from the proximal region around the splenic artery. RNAseq and differentiation trajectory analysis of distinct FSCs showed that Ltbr-deficient cells and perivascular reticular cells (PRCs) from adult spleen exhibited a progenitor phenotype and revealed a closer hierarchical lineage with embryonic mLTo cells. Taken together, our results unveil that embryonic mLTo cells residing in the perivascular niches can give rise to different FSC populations in a LTβR-dependent manner during development.

2019-03-27 | E-MTAB-7097 | biostudies-arrayexpress

Identification of Two Subsets of Murine DC1 Dendritic Cells That Differ by Surface Phenotype, Gene Expression, and Function

Project description:RNAseq of Murine Dendritic Cell Subsets

2021-11-17 | GSE181475 | GEO