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Genome-wide transcriptional analysis of CD8+ brain TRM during CNS autoimmunity

ABSTRACT: Genome-wide transcriptional analysis of CD8+ brain TRM during CNS autoimmunity

PROVIDER: PRJNA783647 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR17038852.fastq.gz	Fastqsanger.gz
	SRR17038853.fastq.gz	Fastqsanger.gz
	SRR17038854.fastq.gz	Fastqsanger.gz
	SRR17038855.fastq.gz	Fastqsanger.gz
	SRR17038856.fastq.gz	Fastqsanger.gz

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Genome-wide transcriptional analysis of CD8+ brain TRM during CNS autoimmunity

Project description:CD8+ TRM are described in autoimmune and chronic inflammatory diseases. However, we still lack knowledge about mechanisms regulating TRM reactivation, in particular in autoimmune diseases. Here, we investigated in a model of TRM-driven central nervous system autoimmunity the transcriptional landscape of self-reactive brain TRM and the requirement of these cells for CD4+ T cell help.

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Molecular signature of brain resident memory CD8+ T cells

Project description:Tissue resident memory (Trm) represent a newly described memory T cell population. We have previously characterized a population of Trm that persists within the brain following acute virus infection. Although capable of providing marked protection against a subsequent local challenge, brain Trm do not undergo recall expansion following dissociation from the tissue. Furthermore, these Trm do not depend on the same survival factors as the circulating memory T cell pool as assessed either in vivo or in vitro. To gain greater insight into this population of cells we compared the gene-expression profiles of Trm isolated from the brain to circulating memory T cells isolated from the spleen following an acute virus infection. Trm displayed altered expression of genes involved in chemotaxis, expressed a distinct set of transcription factors and overexpressed several inhibitory receptors. Cumulatively, these data indicates that Trm are a distinct memory T cell population disconnected from the circulating memory T cell pool and displaying a unique molecular signature which likely results in optimal survival and function within their local environment. 13 samples were analyzed: 5 replicates of memory OT-I CD8+.CD103- T cells isolated from the spleen of mice on day 20 p.i. with VSV-OVA. 5 replicates of memory OT-I CD8+CD103+ T cells isolated from the brain of mice on day 20 p.i. with VSV-OVA; and 3 replicates of memory OT-I.CD8+ CD103- T cells isolated from the brain of mice on day 20 p.i. with VSV-OVA

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Brain-infiltrating CD8+ T cell transcriptional activity after acute infection and during CNS autoimmunity [RNA-Seq]

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Brain-infiltrating CD8+ T cell chromatin accessibility after acute infection and during CNS autoimmunity [ATAC-Seq]

Project description:Brain-infiltrating CD8+ T cell chromatin accessibility after acute infection and during CNS autoimmunity [ATAC-Seq]

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Project description:CD8+ T cells play a pivotal role in eliminating pathogens or tumors, but they equally exert tissue damage in autoimmune or chronic inflammatory diseases. While the mechanisms regulating the differentiation of CD8+ T cells in chronic infections and cancer are just beginning to be unraveled, we still lack knowledge about how this unfolds in autoimmune diseases. Here, we investigated in a model of chronic central nervous system autoimmunity, the transcriptional and epigenetic landscape of autoreactive brain infiltrating CD8+ T cells and how these cells diverge from classical long-lived memory CD8+ T emerging after virus infection.

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Transcriptional profiling of brain CD4+ and CD8+ TRM cells reveals dominant presence in white and grey matter in Multiple Sclerosis (MS)

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