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RNA-seq analysis of ipsilateral rat TGs on day 14 after CCI-ION or sham operation

ABSTRACT: RNA-seq analysis of ipsilateral rat TGs on day 14 after CCI-ION or sham operation

PROVIDER: PRJNA793240 | ENA |

REPOSITORIES: ENA

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RNA-seq analysis of ipsilateral rat TGs on day 14 after CCI-ION or sham operation

Project description:Nerve injury-induced changes in gene expression in primary sensory neurons, such as trigeminal ganglion (TG) neurons, play a critical role in the genesis of neuropathic pain. Therefore, understanding the molecular mechanisms underlying these changes in the TGs following peripheral nerve injury will enable us to develop a new avenue for managing trigeminal-mediated neuropathic pain. Studies have highlighted the involvement of miRNA-mediated modulation in a wide range of diseases, leading to the exploration of miRNA-based therapeutics as a potential treatment strategy. Here, in this RNA-seq database, we have found that microRNA-216a-3p (miR-216a-3p) and miR-32-5p (miR-32-5p), which are downregulated in injured TGs, are novel functional RNAs involved in regulating trigeminal-mediated neuropathic pain. Histone methylation-mediated miRNA downregulation in TG neurons regulates trigeminal neuropathic pain by targeting either STIM1 (H3K27me3/SOX10/miR-216a-3p/STIM1) or Cav3.2 (GR/miR-32-5p/Cav3.2) channels. Moreover, we found that miR-323-3p exhibited the most significant upregulation in the injured TG. Understanding the mechanistic role of the PRMT2/FOXA2/miR-323-3p/Kv2.1 signaling axis in sensory neurons may advance the discovery of novel therapeutic strategies for neuropathic pain.

2025-01-08 | GSE192803 | GEO

DRG from CCI and Seltzer models of neuropathic pain and sham operated animals

Project description:L5 DRG samples from CCI, Seltzer and sham models collected at 14, 21 and 50 days after surgery from ipsilateral hind limb. Note that all replicates are techincal, i.e., there were no biological replicates in this study as samples were pooled for each group Keywords: ordered

2005-05-21 | GSE2636 | GEO

mRNA sequencing of perilesional cortex and ipsilateral thalamus at 3-months after lateral fluid-percussion injury or sham-operation

Project description:At 3-months after after traumatic brain injury, messenger RNA sequencing was performed on samples from ipsilateral thalamus and perilesional cortex of selected rats with the chronic inflammatory endophenotype, and sham-operated controls.

2022-01-26 | GSE192979 | GEO

Small RNA sequencing of perilesional cortex and ipsilateral thalamus at 3-months after lateral fluid-percussion injury or sham-operation

Project description:At 3-months after after traumatic brain injury, small RNA sequencing was performed on samples from ipsilateral thalamus and perilesional cortex of selected rats with the chronic inflammatory endophenotype, and sham-operated controls.

2022-01-26 | GSE192980 | GEO

Transcription profiling of rat dorsal root ganglia from CCI and Seltzer models of neuropathic pain

2007-11-03 | E-GEOD-2636 | biostudies-arrayexpress

mRNA sequencing of perilesional cortex and ipsilateral thalamus at 3-months after lateral fluid-percussion injury or sham-operation

Project description:mRNA sequencing of perilesional cortex and ipsilateral thalamus at 3-months after lateral fluid-percussion injury or sham-operation

| PRJNA794059 | ENA

Small RNA sequencing of perilesional cortex and ipsilateral thalamus at 3-months after lateral fluid-percussion injury or sham-operation

Project description:Small RNA sequencing of perilesional cortex and ipsilateral thalamus at 3-months after lateral fluid-percussion injury or sham-operation

| PRJNA794058 | ENA

Transcriptome Analysis of the Mouse Medial Prefrontal Cortex in Chronic Constriction Injury Model

Project description:The medial prefrontal cortex (mPFC) is critical for both the sensory and emotional /cognitive components of pain. However, the underlying mechanism still remains largely unknown. Here, we examined changes in the transciptomic profiles in the mPFC of mice with chronic pain using RNA sequencing (RNA-seq) technology. We then performed gene expression profiling analysis using data obtained from RNA-seq of 4 different group of mPFC from mice, including ipsilateral part (Left side, the same to our operation part) of mPFC, contralateral part (Right side, opposite to our operation part) of mPFC after CCI (Chronic Constriction Injury of Sciatic Nerve) operation. Combining with naïve mice, at 28 days after operation.

2023-02-16 | GSE225145 | GEO

Lumbar spinal cords: Sham rats vs. chronic constriction injury (CCI) induced chronic neuropathic pain rats

Project description:To investigate the role of DNA methylation in modulating chronic neuropathic pain (NPP), and identify possible target genes of DNA methylation involved in this process. The chronic constriction injury (CCI) induced NPP model was used. The Arraystar Rat RefSeq Promoter Arrays were used to identify the methylation profiles at the genome-wide level in the DNA promoter regions of the lumbar spinal cord in rats 14 Days following CCI surgery. The underlying genes with differential methylation were then identified and submitted to Gene Ontology and pathway analysis. Methyl-DNA immunoprecipitation quantitative PCR (MeDIP-qPCR) and quantitative reverse transcription-PCR (RT‒qPCR) were used to confirm gene methylation and expression.

2022-12-07 | GSE219038 | GEO

Human glioma cells treated with irradiation and/or CCI-779

Project description:Gene expression profiling of human glioma cell line LN-308. Cells were treated with the mTOR inhibitor CCI-779 or irradiated with a single dose of 4 Gy or a combination of both. The objective of this studywas to evaluate CCI-779 as a radio-sensitizing agent and to elucidate the underlying mechanisms. Irradiated, CCI-779-, DMSO- (vehicle control) or combination treated LN-308 samples were hybridized against pooled untreated LN-308 samples as reference (CCI+Irradiation vs. Ref; CCI vs. Ref; DMSO+Irradiation vs Ref; DMSO vs. Ref).Three independent replicates were generated for each treatment and control, respectively.

2011-09-19 | E-GEOD-24232 | biostudies-arrayexpress

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