Project description:To understand the effect of FUS protein knock-down on poly(A+) RNAs profiles, we carried out control and FUS depletion (using synthetic siRNAs) experiments followed by poly(A+) RNA 3'-seq anlyais using lexogen kit (016.024)in HeLa cells
Project description:Efficient TYRP1 knock-down (KD) have been performed in human melanoma cell line expressing a high level of TYRP1 mRNA and protein in order to identify the molecular pathway explaining the decrease of cell proliferation in TYRP1 KD cells.
Project description:To understand how CFIm25, an mRNA 3' processing regulator, affects the global poly(A+) RNAs profiling in hESCs (H9 cell line). We carried out poly(A+)-seq in control (2 samples) and CFIm25 KD (3 samples) hESCs.Using a cutoff value (P<0.05, fold change>1), in total, 577 differentially expressed genes (268 down-regulated genes and 309 up-regulated genes) were found upon CFIm25 KD, and the number of down-regulated genes and up-regulated genes were 84 and 128 respectively if using the cutoff value (P<0.05, fold change>2).
Project description:To identify FUS regulated genes and its dependency of snRNP70, the gene expression profiles in neurons expressing the Gly-rich domain deleted FUS mutant coupled with Snrnp70 gene knock down were analyzed.
Project description:To investigate the function FUS in the regulation of sunitinib-resistance, we used lentiviral transduction to knock down FUS expression in the sunitinib-resistant RCC cells.