Project description:2D IDA protein quantitation of mesenchymal stem cells derived from bone
marrow across five donors. A total of 10 2D LC-MS runs were performed, using cells both not stimulated and following a 20 hour treatment with interferon gamma.
Project description:Mesenchymal stromal cells are a critical component of the bone marrow hematopoietic stem cell niche. In myelofibrosis, these cells are the major source of fibrosis in the bone marrow. We performed gene expression analysis using microarrays to systematically elucidate the mechanisms leading to fibrogenic conversion of these cells.
Project description:Mesenchymal stem cells (MSCs) are involved in tissue homeostasis and repair, and can be impaired in subjects with high fat diet (HFD)-induced metabolic disorders. We used microarrays to detail the global programme of gene expression bone marrow MSCs (BMSCs) underlying HFD-induced progression of metabolic disorders in rat and identified distinct classes of differentiatilly expressed genes during this process.
Project description:Extracellular matrix (ECM) acts as an intrinsic and natural microenvironment of cells, has been shown a pivotal effect on numerous biological processes of MSCs. Especially, MSCs are inclined to differentiate into specific cells lineage commitment when grow on their adaptive and tissue-like microenvironment elasticity. The mechanism still remains elusive on mechanical transduction processes in the microenvironment. In this experiment, human bone marrow mesenchymal stem cells were seeded on substates with different ECM rigidity, and the changes of gene expression were detected by chips, so as to explore the effect of extracellular microenvironment on stem cell differentiation.
Project description:Murine models of myeloid neoplasia show how leukemia infiltration alters the mesenchymal stem and progenitor cells to reinforce malignancy at the expense of healthy hematopoiesis. However, little is known about the bone marrow architecture in humans. We used global gene expression analyses to analyze mesenchymal stem and progenitor cells from AML patients and compared with mesenchymal stem and progenitor cells from the non-leukemic donor.
Project description:Rat lung-resident mesenchymal stem cells (LR-MSCs) were isolated via bronchoalveolar lavage from fibroblast growth factor-10 (FGF-10) pretreated lungs. We characterized the similarity and diversity between LR-MSCs and bone marrow-derived mesenchymal stem cells (BM-MSCs) by transcriptional profiling of these two types of cells. In this dataset, we include the expression data obtained from LR-MSCs and BM-MSCs cultured under similar conditions at passage 5. These data are used to obtain genes that are differentially expressed by these two types of cells. 6 Total samples were analyzed. Both LR-MSCs and BM-MSCs are in three replicates.
Project description:Synovial sarcoma is a rare malignancy characterized by the presence of a specific chromosomal translocation t(X;18) that results in the formation of a fusion protein SYT-SSX. Because it is believed that synovial sarcoma arises from mesenchymal stem or progenitor cells, we used human bone marrow-derived mesenchymal stem cells (MSCs) to determine the changes in gene expression caused by this oncogene in untransformed MSCs. In 2 independent experiments, human bone marrow-derived MSCs were infected with retrovirus carrying either control vector (pOZ) or SYT-SSX2 expression vector. Total cellular RNA was extracted 4 days post-infection and utilized for microarray analysis on Affymetrix arrays.
Project description:Rat lung-resident mesenchymal stem cells (LR-MSCs) were isolated via bronchoalveolar lavage from fibroblast growth factor-10 (FGF-10) pretreated lungs. We characterized the similarity and diversity between LR-MSCs and bone marrow-derived mesenchymal stem cells (BM-MSCs) by transcriptional profiling of these two types of cells. In this dataset, we include the expression data obtained from LR-MSCs and BM-MSCs cultured under similar conditions at passage 5. These data are used to obtain genes that are differentially expressed by these two types of cells.