Ontology highlight
ABSTRACT:
PROVIDER: PRJNA796822 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR17601566.fastq.gz | Fastqsanger.gz | |||
| SRR17601567.fastq.gz | Fastqsanger.gz | |||
| SRR17601568.fastq.gz | Fastqsanger.gz | |||
| SRR17601569.fastq.gz | Fastqsanger.gz | |||
| SRR17601570.fastq.gz | Fastqsanger.gz |
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Project description:We developed a novel approach, m6A-seq, for high-resolution mapping of the transcriptome-wide m6A landscape, based on antibody-mediated capture followed by massively parallel sequencing. Identification of m6A modified sequences in HepG2 cells. HepG2 cells were incubated with either IFNg (200ng/ml) or HGF/SF (10 ng/ml) over night. Stress effects were tested in HepG2 cells by either 30 minutes incubation at 43M-BM-:C (heat shock) or UV irradiation of 0.04 J/cm2 followed by 4 hours of recovery in normal growing conditions prior to harvesting using Trypsin.
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