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RNA-seq Analysis of intestinal tubes from the Unfed and Fed Xenopus laevis tadpoles

ABSTRACT: RNA-seq Analysis of intestinal tubes from the Unfed and Fed Xenopus laevis tadpoles

PROVIDER: PRJNA799304 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR17690155_1.fastq.gz	Fastqsanger.gz
	SRR17690155_2.fastq.gz	Fastqsanger.gz
	SRR17690156_1.fastq.gz	Fastqsanger.gz
	SRR17690156_2.fastq.gz	Fastqsanger.gz
	SRR17690157_1.fastq.gz	Fastqsanger.gz

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RNA-seq Analysis of intestinal tubes from the Unfed and Fed Xenopus laevis tadpoles

Project description:In this study, we assessed nutrient-dependent organ morphogenesis in Xenopus laevis. We performed the RNA-seq analysis of the gastrointestinal (GI) tract for both fed and unfed tadpoles to identify feeding-dependent inter-organ transmitters secreted from GI tract post-prandially during development. We found that one of the GI hormones, glucose-dependent insulinotropic polypeptide (GIP), is required for thyroid morphogenesis.

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Project description:Premetamorphic Xenopus laevis tadpole tail respond to thyroid hormone by resorption. The goal of this experiment is to identify the genes involved in the TH-induced resorption tadpole tail and compare it to TH-induced proliferation and differentiation program in tadpole limb and brain. Xenopus tadpoles (NF54) were treated with 100 nM T3 in 0.1 x MMR for another 24h and 48h or without T3 for 48h (control group). NF 61 tadpoles were in 0.1 X MMR till they reached NF stage 62. The tails were dissected after the experiment. Keywords: development or differentiation design,organism part comparison design,reference design,replicate design,time series design

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