Project description:A Platichthys flesus cDNA microarray (GENIPOL) comprised of 12,700 clones, was used to determine and characterize gene expression profiles of flounders (Platichthys flesus) exposed to polybrominated diphenyl ethers (pentamix).
Project description:A Platichthys flesus cDNA microarray (GENIPOL) comprised of 12,700 clones, was used to determine and characterize gene expression profiles of environmentally sampled flounders from sites of different pollution status in Northern Europe.
Project description:Using the GENIPOL flounder cDNA microarray, we assessed the temporal transcriptomic responses of Platichthys flesus to model toxicants over a 16 day timespan. Immature fish were treated by intraperitoneal injection with cadmium chloride (50 micrograms/kg in saline), 3-methylcholanthrene (25mg/kg in olive oil), Aroclor 1254 (50mg/kg in olive oil), tert-butyl-hydroperoxide (5mg/kg in saline), lindane (25 mg/kg in olive oil), perfluoro-octanoic acid (100mg/kg in olive oil), olive oil or saline (0.9%). Hepatic gene expression changes were determined 1,2,4,8 and 16 days post-injection in comparison with time-matched carrier controls.
Project description:Proteomics of Platichthys flesus livers after sampling different estuarine sites to describe key proteins in response to environmental stresses.
Project description:Proteomics of livers from Platichthys flesus after encagement in different estuarine sites for describing key molecular players in response to environmental stresses.
Project description:Proteomics of livers from Platichthys flesus caught in the Canche estuary but having undergone a 1-month encagement in three estuarine sites for describing molecular players in response to encagement and environmental stresses.
Project description:Proteomics of livers from Platichthys flesus after encagement in the Seine estuary at day 15 and day 30 after the fire of the Lubrizol and NL-logistique plants in Rouen, France, and in a control estuary for describing key molecular players in response to industrial pollutants.
