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ABSTRACT: RNA-seq of Platymeris biguttatus salivary gland

PROVIDER: PRJNA801928 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR17828248_1.fastq.gz	Fastqsanger.gz
	SRR17828248_2.fastq.gz	Fastqsanger.gz
	SRR17828249_1.fastq.gz	Fastqsanger.gz
	SRR17828249_2.fastq.gz	Fastqsanger.gz
	SRR17828250_1.fastq.gz	Fastqsanger.gz

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Project description:Investigating how the Wnt-driven Mll1 epigenome regulates salivary gland and head and neck cancer. We performed mRNA-seq and ChIP-seq of H3K4me1, me2 and me3 on mouse salivary gland cancer cells that are kept in two different growth conditions, adherent culture and non-adherent sphere culture. Mouse salivary gland cancer cells were isolated from salivary gland of transgenic mouse that harbor K14-Cre-induced Wnt/β-catenin gain-of-function and Bmpr1a loss-of-function mutations. Anti-H3K4me1 (C15410194), -me2 (C15410035) and -me3 (C15410003-50) antibodies were purchased from Diagenode. ChIP-seq was performed according to the protocols provided by Diagenode using the iDeal ChIP-seq kit for histones and the iDeal library preparation kit. For mRNA-seq, mRNA was extracted according to the standard TRIzol protocol (Invitrogen) and subjected to library preparation using the TruSeq stranded mRNA library preparation kit. Sequencing was performed with the TruSeq SBS Kit v3-HS (2 X 200 cycles) on an Illumina HiSeq 2000 sequencer.

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Proteotranscriptomic Analysis and Toxicity Assay Suggest the Functional Distinction between Venom Gland Chambers in Twin-Spotted Assassin Bug, Platymeris biguttatus

Project description:Assassin bugs are one of the diversified groups of venomous predatory insects. Their venom is secreted from three different lumens of salivary gland and can lead to paralysis, lethality and liquidation of the prey, respectively. Nevertheless, the venom components responsible for these effects are not clear. In this study, we use transcriptomics and proteomics to determine the effective salivary protein components in the separate lumen of salivary gland from the assassin bug Platymeris biguttatus, and to conduct toxicological analysis on the function of the salivary gland compartments. Our study sheds light on the functional cooperation between different salivary gland lumens of assassin bugs, and will further the understanding of physiological adaptations of venom-based predation and defense in venomous insects

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