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Morphine Re-arranges Chromatin Spatial Architecture of Primate Cortical Neuron [HiC]

ABSTRACT: Morphine Re-arranges Chromatin Spatial Architecture of Primate Cortical Neuron [HiC]

PROVIDER: PRJNA803901 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR17901500_1.fastq.gz	Fastqsanger.gz
	SRR17901500_2.fastq.gz	Fastqsanger.gz
	SRR17901501_1.fastq.gz	Fastqsanger.gz
	SRR17901501_2.fastq.gz	Fastqsanger.gz
	SRR17901502_1.fastq.gz	Fastqsanger.gz

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Morphine Re-arranges Chromatin Spatial Architecture of Primate Cortical Neuron

Project description:Morphine Re-arranges Chromatin Spatial Architecture of Primate Cortical Neuron

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Morphine Re-arranges Chromatin Spatial Architecture of Primate Cortical Neuron [HiC]

Project description:Purpose:The goals of this study are to investigate the 3D chromatin architecture of cortical neurons in rhesus monkeys treated with morphine for 90 days using digestion-ligation-only high-throughput chromosome conformation capture technology combined with transcriptome profiling (RNA-seq).

2023-09-12 | GSE196209 | GEO

Morphine Re-arranges Chromatin Spatial Architecture of Primate Cortical Neuron [RNA-seq]

Project description:Morphine Re-arranges Chromatin Spatial Architecture of Primate Cortical Neuron [RNA-seq]

| PRJNA803900 | ENA

Morphine Re-arranges Chromatin Spatial Architecture of Primate Cortical Neuron [RNA-seq]

Project description:Purpose: The goals of this study are to investigate the 3D chromatin architecture of cortical neurons in rhesus monkeys treated with morphine for 90 days using digestion-ligation-only high-throughput chromosome conformation capture technology combined with transcriptome profiling (RNA-seq).

2023-09-12 | GSE196210 | GEO

Morphine Re-arranges Chromatin Spatial Architecture of Primate Cortical Neuron

Project description:This SuperSeries is composed of the SubSeries listed below.

2023-09-12 | GSE196212 | GEO

Morphine Re-arranges Chromatin Spatial Architecture of Primate Cortical Neurons.

Project description:The expression of linear DNA sequence is precisely regulated by the three-dimensional (3D) architecture of chromatin. Morphine-induced aberrant gene networks of neurons have been extensively investigated; however, how morphine impacts the 3D genomic architecture of neurons is still unknown. Here, we applied digestion-ligation-only high-throughput chromosome conformation capture (DLO Hi-C) technology to investigate the effects of morphine on the 3D chromatin architecture of primate cortical neurons. After receiving continuous morphine administration for 90 days on rhesus monkeys, we discovered that morphine re-arranged chromosome territories, with a total of 391 segmented compartments being switched. Morphine altered over half of the detected topologically associated domains (TADs), most of which exhibited a variety of shifts, followed by separating and fusing types. Analysis of the looping events at kilobase-scale resolution revealed that morphine increased not only the number but also the length of differential loops. Moreover, all identified differentially expressed genes from the RNA sequencing data were mapped to the specific TAD boundaries or differential loops, and were further validated for changed expression. Collectively, an altered 3D genomic architecture of cortical neurons may regulate the gene networks associated with morphine effects. Our finding provides critical hubs connecting chromosome spatial organization and gene networks associated with the morphine effects in humans.

| S-EPMC10787020 | biostudies-literature

m6a modifications following morphine exposure or Alkbh5 siRNA in primary cortical neuron cultures

Project description:m6a modified RNA and unmodified RNA were quantified in primary neuronal cultures following either siRNA knockdown of Alkbh5 or chronic morphine exposure for 3 days. The goal was to identify mRNA that have m6A modifications following one or more treatment conditions.

2024-09-01 | GSE243685 | GEO

m6a modifications following morphine exposure or Alkbh5 siRNA in primary cortical neuron cultures

Project description:m6a modifications following morphine exposure or Alkbh5 siRNA in primary cortical neuron cultures

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Morphine PVT TRAPSeq

Project description:Using an oral self-administration paradigm, we have seen morphine increase neural activity in the Paraventricular Nucleus of the Thalamus (PVT). Morphine binds primarily to Mu Opioid Receptors (MORs), which are highly expressed in the PVT. Translating Ribosome Affinity Purification (TRAP)-Sequencing was conducted on PVT neurons that express MORs

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Morphine PVT TRAPSeq

Project description:Morphine PVT TRAPSeq

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