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Morphine Re-arranges Chromatin Spatial Architecture of Primate Cortical Neuron [HiC]

ABSTRACT: Purpose:The goals of this study are to investigate the 3D chromatin architecture of cortical neurons in rhesus monkeys treated with morphine for 90 days using digestion-ligation-only high-throughput chromosome conformation capture technology combined with transcriptome profiling (RNA-seq).

ORGANISM(S): Macaca mulatta

PROVIDER: GSE196209 | GEO | 2023/09/12

REPOSITORIES: GEO

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Morphine Re-arranges Chromatin Spatial Architecture of Primate Cortical Neuron [RNA-seq]

Project description:Purpose: The goals of this study are to investigate the 3D chromatin architecture of cortical neurons in rhesus monkeys treated with morphine for 90 days using digestion-ligation-only high-throughput chromosome conformation capture technology combined with transcriptome profiling (RNA-seq).

2023-09-12 | GSE196210 | GEO

Morphine Re-arranges Chromatin Spatial Architecture of Primate Cortical Neuron [HiC]

Project description:Morphine Re-arranges Chromatin Spatial Architecture of Primate Cortical Neuron [HiC]

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Morphine Re-arranges Chromatin Spatial Architecture of Primate Cortical Neuron

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Morphine Re-arranges Chromatin Spatial Architecture of Primate Cortical Neuron

Project description:This SuperSeries is composed of the SubSeries listed below.

2023-09-12 | GSE196212 | GEO

Morphine Re-arranges Chromatin Spatial Architecture of Primate Cortical Neuron [RNA-seq]

Project description:Morphine Re-arranges Chromatin Spatial Architecture of Primate Cortical Neuron [RNA-seq]

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Morphine Alters Systemic Responses to SIV Infection of Rhesus Monkeys and Changes Brain Macrophage and Microglia Gene Expression Favoring Neuropathogenesis

Project description:In this study, we investigated longitudinal changes in response to SIV infection of rhesus monkeys in the presence of morphine, compared to animals administered saline, before and after cART treatment. Using single-cell and single-nucleus RNA sequencing (scRNA-seq and snRNA-seq), we examined the effect of morphine on brain-resident macrophages and microglia in these SIV-infected, cART-suppressed animals to better understand the factors responsible for the morphine-induced increased CNS reservoir and the role of opioids in neuropathogenesis.

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Morphine Re-arranges Chromatin Spatial Architecture of Primate Cortical Neurons.

Project description:The expression of linear DNA sequence is precisely regulated by the three-dimensional (3D) architecture of chromatin. Morphine-induced aberrant gene networks of neurons have been extensively investigated; however, how morphine impacts the 3D genomic architecture of neurons is still unknown. Here, we applied digestion-ligation-only high-throughput chromosome conformation capture (DLO Hi-C) technology to investigate the effects of morphine on the 3D chromatin architecture of primate cortical neurons. After receiving continuous morphine administration for 90 days on rhesus monkeys, we discovered that morphine re-arranged chromosome territories, with a total of 391 segmented compartments being switched. Morphine altered over half of the detected topologically associated domains (TADs), most of which exhibited a variety of shifts, followed by separating and fusing types. Analysis of the looping events at kilobase-scale resolution revealed that morphine increased not only the number but also the length of differential loops. Moreover, all identified differentially expressed genes from the RNA sequencing data were mapped to the specific TAD boundaries or differential loops, and were further validated for changed expression. Collectively, an altered 3D genomic architecture of cortical neurons may regulate the gene networks associated with morphine effects. Our finding provides critical hubs connecting chromosome spatial organization and gene networks associated with the morphine effects in humans.

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m6a modifications following morphine exposure or Alkbh5 siRNA in primary cortical neuron cultures

Project description:m6a modified RNA and unmodified RNA were quantified in primary neuronal cultures following either siRNA knockdown of Alkbh5 or chronic morphine exposure for 3 days. The goal was to identify mRNA that have m6A modifications following one or more treatment conditions.

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Multi-organ proteomics of rhesus macaque for prototype proteome bank of non–human primate

Project description:Though the rhesus monkey is one of the most valuable non-human primate animal models for various human diseases because of its manageable size and genetic and proteomic similarities with humans, proteomic research using rhesus monkeys still remains challenging due to the lack of a complete protein sequence database and sufficient proteomic information. In this project, proteomic profiling of multiple organ tissues, 9 male and 11 female were performed in an automated, high-throughput manner employing annotated UniProtKB human database. Based on the success of this alternative interpretation of MS data, the list of proteins identified from total 12 organs of male and female subjects will benefit future rhesus monkey proteome research.

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