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Multiple RSV strains infecting HEp-2 and A549 cells reveal cell line-dependent differences in resistance to RSV infection

ABSTRACT: Multiple RSV strains infecting HEp-2 and A549 cells reveal cell line-dependent differences in resistance to RSV infection

PROVIDER: PRJNA804555 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR17950700_1.fastq.gz	Fastqsanger.gz
	SRR17950700_2.fastq.gz	Fastqsanger.gz
	SRR17950701_1.fastq.gz	Fastqsanger.gz
	SRR17950701_2.fastq.gz	Fastqsanger.gz
	SRR17950702_1.fastq.gz	Fastqsanger.gz

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Multiple RSV strains infecting HEp-2 and A549 cells reveal cell line-dependent differences in resistance to RSV infection

Project description:A few transformed cell lines and two historic strains have been extensively used to study respiratory syncytial virus (RSV). We report a thorough molecular and cell biological characterization of HEp-2 and A549 cells infected with four strains of RSV representing both major subgroups as well as historic and more contemporary genotypes -- [RSV/A/Tracy (GA1), RSV/A/Ontario (ON), RSV/B/18537 (GB1), RSV/B/Buenos Aires (BA)] -- via measurements of viral replication kinetics and viral gene expression, immunofluorescence-based imaging of gross cellular morphology and cell-associated RSV, and measurements of host response including transcriptional changes and levels of secreted cytokines and growth factors. Our findings strongly suggest 1) the existence of a conserved difference in gene expression between RSV subgroups A and B; 2) the A549 cell line is a more stringent and natural host of replicating RSV than the HEp-2 cell line; and 3) consistent with previous studies, determining the full effects of viral genetic variation in RSV pathogenesis requires model systems as tractable as transformed cell lines but better representative of the human host.

2022-02-10 | GSE196385 | GEO

Multiple Respiratory Syncytial Virus (RSV) Strains Infecting HEp-2 and A549 Cells Reveal Cell Line-Dependent Differences in Resistance to RSV Infection.

Project description:Respiratory syncytial virus (RSV) is a leading cause of pediatric acute respiratory infection worldwide. There are currently no approved vaccines or antivirals to combat RSV disease. A few transformed cell lines and two historic strains have been extensively used to study RSV. Here, we reported a thorough molecular and cell biological characterization of HEp-2 and A549 cells infected with one of four strains of RSV representing both major subgroups as well as historic and more contemporary genotypes (RSV/A/Tracy [GA1], RSV/A/Ontario [ON], RSV/B/18537 [GB1], and RSV/B/Buenos Aires [BA]) via measurements of viral replication kinetics and viral gene expression, immunofluorescence-based imaging of gross cellular morphology and cell-associated RSV, and measurements of host response, including transcriptional changes and levels of secreted cytokines and growth factors. IMPORTANCE Infection with the respiratory syncytial virus (RSV) early in life is essentially guaranteed and can lead to severe disease. Most RSV studies have involved either of two historic RSV/A strains infecting one of two cell lines, HEp-2 or A549 cells. However, RSV contains ample variation within two evolving subgroups (A and B), and HEp-2 and A549 cell lines are genetically distinct. Here, we measured viral action and host response in both HEp-2 and A549 cells infected with four RSV strains from both subgroups and representing both historic and more contemporary strains. We discovered a subgroup-dependent difference in viral gene expression and found A549 cells were more potently antiviral and more sensitive, albeit subtly, to viral variation. Our findings revealed important differences between RSV subgroups and two widely used cell lines and provided baseline data for experiments with model systems better representative of natural RSV infection.

| S-EPMC9006923 | biostudies-literature

Alteration in gene expression in the lung following neonatal RSV infection

Project description:mRNA expression data from BALB/c mice which were infected intranasally with Respiratory Syncytial Virus (or Hep-2 cell lysate control) at 1 week old and challenged with PBS or house dust mite (HDM) extract as adults. Experimental groups: RH – neonatal RSV, adult HDM, RP – neonatal RSV, adult PBS, HH – neonatal Hep-2, adult HDM and HP – neonatal Hep-2, adult PBS.

2019-03-19 | E-MTAB-3654 | biostudies-arrayexpress

Respiratory Syncytial virus (RSV) directly binds host microRNAs and de-regulates host gene targets [RNA-seq]

Project description:To investigate RNA expression changes in RSV infection, we performed total RNA-seq from Mock and RSV-infected A549 cells In addition, we investigated the association of RNAs with AGO2 by performing AGO2 immunoprecipitation and total RNA-seq of AGO2-bound RNAs in Mock and RSV-infected A549 cells

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Uninfected and human respiratory syncytial virus (RSV)-infected A549 cells, analysed using bottom-up mass spectrometry

Project description:Proteomic data from uninfected and human respiratory syncytial virus (RSV) infected A549 cells (24h post infection; four biological replicate sets). Data is related to the fractionation-free workflow described in Dave et al. (2014) (PMID: 25106423). Data is processed using MaxQuant (version 1.3.0.5).

2016-08-08 | MSV000080032 | MassIVE

RSV vs BSA

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2014-06-21 | E-GEOD-58425 | biostudies-arrayexpress

Uninfected and human respiratory syncytial virus (RSV)-infected A549 cells, analysed using in-solution protein IEF followed by bottom-up mass spectrometry

Project description:Proteomic data from uninfected and human respiratory syncytial virus (RSV)-infected A549 cells (24h post infection; five paired biological replicate sets). Data is related to the in-solution protein IEF workflow described in Dave et al. (2014) (PMID: 25106423). Data processed using MaxQuant (version 1.3.0.5).

2016-01-12 | MSV000079453 | MassIVE

Global hepatic transcript data from LRH-1 hep+/+ and LRH-1 hep-/- livers

2014-07-11 | GSE59305 | GEO

Expression analysis of sk-hep-1(sk) cell and sk-hep-1-sh EZH2(sks) cell

Project description:Investigation of EZH2 knocking down on whole genome gene expression level changes in sk-hep-1 compared to sk-hep-1-sh EZH2.

2015-08-13 | GSE68863 | GEO

Global hepatic transcript data from LRH-1 hep+/+ and LRH-1 hep-/- livers

Project description:Transcript data from LRH-1 hep+/+ and LRH-1 hep-/- livers from mice fed ad libitum and sacrificed at 7 am We used microarrays to detail the global programme of gene expression underlying hepatic function in ad libitum fed mice. 17-19-week-old mice were fed ad libitum, sacrificed at 7 am, and livers snap-frozen in liquid nitorgen for RNA extraction and hybridization on Affymetrix microarrays.

2014-07-11 | E-GEOD-59305 | biostudies-arrayexpress

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