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Quality assurance of hematopoietic stem cells by macrophages determines stem cell clonality [macrophages]

ABSTRACT: Quality assurance of hematopoietic stem cells by macrophages determines stem cell clonality [macrophages]

PROVIDER: PRJNA805357 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR17982193_1.fastq.gz	Fastqsanger.gz
	SRR17982193_2.fastq.gz	Fastqsanger.gz
	SRR17982194_1.fastq.gz	Fastqsanger.gz
	SRR17982194_2.fastq.gz	Fastqsanger.gz

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Quality assurance of hematopoietic stem cells by macrophages determines stem cell clonality

Project description:There are no described quality assurance mechanisms for newly formed stem cells. We observed intimate interactions between macrophages and blood stem cells in zebrafish embryos. Stressed stem cells were marked by surface Calreticulin, which stimulates macrophage interaction as an eat me signal. Macrophage-stem cell interactions either lead to removal of cytoplasmic material and stem cell proliferation or resulted in complete stem cell engulfment. Calreticulin knock down or embryonic macrophage depletion reduced the number of stem cell clones into adulthood. Our work supports a model in which embryonic macrophages determine hematopoietic clonality by monitoring stem cell quality.

2022-02-04 | MSV000088780 | MassIVE

Quality assurance of hematopoietic stem cells by macrophages determines stem cell clonality [macrophages]

Project description:Tissue-specific stem cells persist for a lifetime and can differentiate to maintain homeostasis or transform to initiate cancer. Despite their importance, there are no described quality assurance mechanisms for newly formed stem cells. We observed intimate and specific interactions between macrophages and blood stem cells in zebrafish embryos. Stressed stem cells were marked by surface Calreticulin, which stimulates macrophage interaction as an “eat me” signal. Macrophage-stem cell interactions either lead to removal of cytoplasmic material and stem cell proliferation or resulted in complete stem cell engulfment. Using cellular barcoding, we found that calreticulin knock-down or embryonic macrophage depletion substantially reduced the number of stem cell clones into adulthood. Our work supports a model in which embryonic macrophages determine hematopoietic clonality by monitoring stem cell quality.

2022-10-19 | GSE196551 | GEO

Quality assurance of hematopoietic stem cells by macrophages determines stem cell clonality [HSCP]

2022-10-19 | GSE196552 | GEO

Quality assurance of hematopoietic stem cells by macrophages determines stem cell clonality

Project description:Quality assurance of hematopoietic stem cells by macrophages determines stem cell clonality

| PRJNA805354 | ENA

Quality assurance of hematopoietic stem cells by macrophages determines stem cell clonality [HSCP]

Project description:Quality assurance of hematopoietic stem cells by macrophages determines stem cell clonality [HSCP]

| PRJNA805356 | ENA

Quality assurance of hematopoietic stem cells by macrophages determines stem cell clonality.

Project description:Tissue-specific stem cells persist for a lifetime and can differentiate to maintain homeostasis or transform to initiate cancer. Despite their importance, there are no described quality assurance mechanisms for newly formed stem cells. We observed intimate and specific interactions between macrophages and nascent blood stem cells in zebrafish embryos. Macrophage interactions frequently led to either removal of cytoplasmic material and stem cell division or complete engulfment and stem cell death. Stressed stem cells were marked by surface Calreticulin, which stimulated macrophage interactions. Using cellular barcoding, we found that Calreticulin knock-down or embryonic macrophage depletion reduced the number of stem cell clones that established adult hematopoiesis. Our work supports a model in which embryonic macrophages determine hematopoietic clonality by monitoring stem cell quality.

| S-EPMC9524573 | biostudies-literature

Quality assurance of hematopoietic stem cells by macrophages determines stem cell clonality

Project description:This SuperSeries is composed of the SubSeries listed below.

2022-10-19 | GSE196553 | GEO

A radical switch in clonality reveals the formation of a stem cell niche in the epiphyseal growth plate

Project description:A radical switch in clonality reveals the formation of a stem cell niche in the epiphyseal growth plate

2019-01-03 | GSE113982 | GEO

IFN gamma signaling in cytotoxic T cells restricts antitumor responses by inhibiting the maintenance and clonality of intra-tumoral stem-like T cells

Project description:IFN gamma signaling in cytotoxic T cells restricts antitumor responses by inhibiting the maintenance and clonality of intra-tumoral stem-like T cells

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Time-course expression data during assurance of the chronological longevity by caloric restriction in budding yeast

Project description:Caloric restriction (CR) is the only non-genetic intervention to retard aging and increase longevity in a variety of species. It is important to understand the fundamental mechanism by which CR extends lifespan that remains elusive. Owing to well-established genomic tools and convenience of culture system, we used a single cell organism, Saccharomyces cerevisiae, to clarify the mechanisms of CR. In order to identify genes responsible for CR-mediated longevity, we performed microarray experiments across the longevity assurance time-points. Since the CR-treated cells obtained the longevity potential around 12 hours after inoculation and the strength of potential gradually increased up to 48 hours, we concluded that the changes across these time-points must be a critical need for assurance of longevity by CR. For preparation of total RNA, yeast cells under control (2% glucose) and CR (0.5% glucose) conditions were harvested at 12h, 18h, 24h and 48h after inoculation. After total RNA extraction, we performed Affymetrix Yeast GeneChip 2.0 array for biological triplicate repeats according to the manufacturer's instructions.

2013-12-31 | E-GEOD-41860 | biostudies-arrayexpress

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