Ontology highlight
ABSTRACT:
PROVIDER: PRJNA806977 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:We used single cell RNA-seq to comprehensively map raphidophyte Heterosigma akashiwo to grow under present and future ocean conditions. We unveiled several unique types of metabolic changes, signaling pathways, and regulons associated with these cells.
2023-01-20 | GSE223198 | GEO
Project description:Heterosigma akashiwo transcriptome
| PRJDB15108 | ENA
Project description:Chlamydomonas, a green algae, is known to respond to changes in light intensity by altering its protein expression. In low light conditions, Chlamydomonas increases the expression of photosynthetic genes and the proteins they encode, including the light-harvesting complexes and the reaction center proteins. This allows the algae to maximize its ability to capture light and perform photosynthesis efficiently. On the other hand, under high light conditions, Chlamydomonas reduces the expression of these photosynthetic genes to avoid photoinhibition and damage to the photosynthetic machinery. Instead, it increases the expression of stress response genes and the corresponding proteins, such as antioxidant enzymes, which protect the algae from the harmful effects of excess light. In addition, Chlamydomonas also modulates its expression of other genes and proteins, such as those involved in carbon and nitrogen metabolism, in response to changes in light intensity. This helps the algae to maintain a balance between energy production and utilization, ensuring its survival and growth under different light conditions. Overall, the ability of Chlamydomonas to modulate its protein expression in response to changes in light intensity is an important mechanism for adapting to its environment and ensuring its survival and growth.
2023-02-13 | PXD040080 | JPOST Repository
Project description:Proteins secreted by marine cyanobacterium Synechococcus under phosphorus stress is largely uncharacterized. This dataset characterizes the exoproteins for both an open ocean (WH8102) and coastal (WH5701) Synechococcus strain and were collected as part of the study "Dissolved organic phosphorus bond-class utilization by Synechococcus". Study Abstract: Dissolved organic phosphorus (DOP) contains compounds with phosphoester (P-O-C), phosphoanhydride (P-O-P), and phosphorus-carbon (P-C) bonds. Despite DOP’s importance as a nutritional source for marine microorganisms, the bioavailability of each bond-class to the widespread cyanobacterium Synechococcus remains largely unknown. This study evaluates bond-class specific DOP utilization by cultures of an open ocean and a coastal ocean Synechococcus strain. Both strains exhibited comparable growth rates when provided phosphate, short-chain and long-chain polyphosphate (P-O-P), adenosine 5’-triphosphate (P-O-C and P-O-P), and glucose-6-phosphate (P-O-C) as the phosphorus source. However, growth rates on phosphomonoester adenosine 5’-monophosphate (P-O-C) and phosphodiester bis(4-methylumbelliferyl) phosphate (C-O-P-O-C) varied between strains, and neither strain grew on selected phosphonates. Consistent with the growth measurements, both strains preferentially hydrolyzed 3-polyphosphate, followed by adenosine 5’-triphosphate, and then adenosine 5’-monophosphate. The strains’ exoproteome contained phosphorus hydrolases, which combined with enhanced cell-free hydrolysis of 3-polyphosphate and adenosine 5’-triphosphate under phosphate deficiency, suggests active mineralization of short-chain polyphosphate by Synechococcus’ exoproteins. Synechococcus alkaline phosphatases presented broad substrate specificities, including activity towards short-chain polyphosphate, with varying affinities between the two strains. Collectively, these findings underscore the potentially significant role of compounds with phosphoanhydride bonds in Synechococcus phosphorus nutrition, thereby expanding our understanding of microbially-mediated DOP cycling in marine ecosystems.
2024-07-15 | PXD051991 | Pride