Project description:Germ cell specification is an elusive biological event, as it occurs in a small group of cells in the very early embryo (E6.5). In vitro differentiation of naive mouse Embryonic Stem Cells (mESCs) to mouse Primordial Germ Cell like cells (mPGCLCs) allows the mechanistic investigation of germ cell specification. The goal of this study is to profile of gene expression via single cell RNA sequencing throughout the differentiation protocol. In particular, this project aims at examining the transcriptional activation of the molecular networks that direct the epigenetic reprogramming of mPGCLCs.
Project description:EpiSCs established from murine epiblast were differentiated to posterior primitive streak (PPS) and analyzed using scRNA-seq to investigate identity and homogeneity of the resulting population.
Project description:Mouse ES cells were differentiated for 6 days. Undifferentiated cells (d0) were compared to cells harvested at 24 hour timepoints (d1-d6).
Project description:Induced pluripotent stem cells (iPSC) derived from fibroblasts of two healthy individuals were differentiated into NPC. Cells were profiled by scRNA-seq.