Project description:In this experiment, we studied how human beta defensin-2 (hBD-2) affects gene expression of T cells. Murine splenic C57BL/6 CD4/CD8 T cells were stimulated with anti-CD3/anti-CD28 beads and hBD-2 or vehicle (PBS) control for 48h before RNA isolation
Project description:Wildtype (WT) BALB/c mice were treated with allogeneic hematopoietic cell transplantation (allo-HCT) in order to induce acute GVHD. C57BL/6 mice were used bone marrow (BM) and T cell donor mice. We studied the effect of oral hBD-2 treatment versus vehicle (PBS) control treatment on the gene expression in the colon, as main GVHD target organ.