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Transcriptome analysis of aortas from aged WT and Sirt2KO mice

ABSTRACT: Transcriptome analysis of aortas from aged WT and Sirt2KO mice

PROVIDER: PRJNA821187 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR18517614_1.fastq.gz	Fastqsanger.gz
	SRR18517614_2.fastq.gz	Fastqsanger.gz
	SRR18517615_1.fastq.gz	Fastqsanger.gz
	SRR18517615_2.fastq.gz	Fastqsanger.gz
	SRR18517616_1.fastq.gz	Fastqsanger.gz

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Transcriptome analysis of aortas from aged WT and Sirt2KO mice

Project description:We analyzed the transcriptome of aortas from aged WT and Sirt2KO mice by bulk RNA-seq

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Transcriptome analaysis of Bcl11b-deficient aortas

Project description:B-cell leukemia 11b (BCL11B) is a transcription factor known as an essential regulator of T lymphocytes and neuronal development during embryogenesis. A genome-wide association study (GWAS) showed that a gene desert region downstream of BCL11B, known to function as a BCL11B enhancer, harbors single nucleotide polymorphisms (SNPs) associated with increased arterial stiffness. However, a role for BCL11B in the adult cardiovascular system is unknown. Based on these human findings, we sought to examine the relation between BCL11B and arterial function. Here we report that BCL11B is expressed in the vascular smooth muscle (VSM) where it regulates vascular stiffness. RNA sequencing of aortas from WT and Bcl11b null mice (BSMKO) identified the cyclic guanosine monophosphate (cGMP)-protein kinase G (PKG) as the most significant differentially regulated signaling pathway in BSMKO compared to WT mice. BSMKO aortas showed decreased levels of PKG1, increased levels of Ca++-calmodulin-dependent serine/threonine phosphatase calcineurin (PP2B) and their common phosphorylation target, vasodilator-stimulated phosphoprotein (pVASPS239), a regulator of cytoskeletal actin rearrangements. Decreased pVASPS239 in BSMKO aortas was associated with increased actin polymerization (F/G actin ratio). Functionally, aortic force, stress, wall tension and stiffness, measured ex vivo in organ baths, were increased in BSMKO aortas, and BSMKO mice had increased pulse wave velocity, the in vivo index of arterial stiffness. Despite having no effect on blood pressure or microalbuminuria, increased arterial stiffness in BSMKO mice was associated with increased incidence of cerebral microbleeds compared to age-matched WT littermates. In conclusion, we have identified VSM BCL11B as a crucial regulator of aortic smooth muscle function and a potential therapeutic target for vascular stiffness.

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Single cell RNA sequencing analysis for atherosclerotic aortas in Oasl1/Apoe WT and Oasl1/Apoe DKO mice

Project description:We have applied 10X single-cell RNA sequencing (scRNA-seq) technique to examine the cell type specific transcriptomes of heterogeneous cell populations in atherosclerotic aortas isolated from Oasl1+/+Apoe-/- and Oasl1-/-Apoe-/- mice.

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Single guide RNA CRISPR /Cas(sg) editing 3 nucleotides and prime editing (pe) single nucleotide of CArG element in Tspan2 promoter effect on transcriptome in aortas

Project description:Aortas from sgCArG WT versus sg CArG homozygous mice at 12 wks and peCArGWT and peCArG homozygenous mutant mice at 8 weeks were isolated for total RNA extraction. RNA samples were then subjected to deeping sequencing to profile gene expression in aortas.

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Single cell RNA sequencing analysis for atherosclerotic aortas in Oasl1/Apoe WT and Oasl1/Apoe DKO mice

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olfactory proteomics in aged Tg2576 mice

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Aortas of mice on high fat diet

Project description:Expression profiles in aortas isolated from mouse strains C3H.2/HeJ and C57.2Bl/6. Mice were were either on a high fat diet or normal diet for 0, 4, 10, 24, or 40 weeks. Keywords: other

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Project description:Transcriptional profiling of mouse aortas comparing control wild-type animals with aortas from three different Plk1(+/-) mice. Goal was to determine the effects of Plk1 haploinsufficiency in aorta expression profiles.

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