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RNA-sequencing uncovers transcriptomic changes in retinal tissues after vitreous substitution with a thermogelling polymer

ABSTRACT: RNA-sequencing uncovers transcriptomic changes in retinal tissues after vitreous substitution with a thermogelling polymer

PROVIDER: PRJNA824789 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR18685671_1.fastq.gz	Fastqsanger.gz
	SRR18685671_2.fastq.gz	Fastqsanger.gz
	SRR18685672_1.fastq.gz	Fastqsanger.gz
	SRR18685672_2.fastq.gz	Fastqsanger.gz
	SRR18685673_1.fastq.gz	Fastqsanger.gz

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Similar Datasets

RNA-sequencing uncovers transcriptomic changes in retinal tissues after vitreous substitution with a thermogelling polymer

Project description:We developed a thermogelling polymer with intrinsic anti-scarring properties on retinal cells in-vitro. To study its mechanism of action in-vivo, this thermogelling polymer was injected into the rabbit eye as a vitreous substitute and the retinal tissues were harvested for RNA-sequencing to understand transcriptomic changes induced by the polymer.

2022-04-09 | GSE200476 | GEO

RNA-sequencing uncovers transcriptomic changes induced by a multiblock thermogelling polymer on healthy and disease-modeled retinal pigment epithelial cells

Project description:Human embryonic stem-cell derived retinal pigment epithelial cells were co-stimulated with TNF-α and TGF-β1 to model a retinal scarring complication, proliferative vitreoretinopathy. Thermogelling polymer prevented this scarring complication. To understand the polymer-cell interaction and ultimately the mechanism underlying it's anti-scarring activity, RNA-Seq was performed.

2022-03-09 | GSE176513 | GEO

Dual-function thermogelling polymers as effective endotamponade for retinal detachment repair and reassembly of vitreous-like body in-situ

Project description:Endo-tamponade agents are crucial surgical adjuncts for vitreo-retinal surgery. Current clinical agents provide internal tamponade through buoyancy forces requiring the need for prolonged facing down post-operative positioning. Other disadvantages include temporary loss of vision, raised intra-ocular pressure, cataract formation and a need for an additional removal surgery. Therein lies an unmet clinical need to develop alternative biomaterials to overcome these key challenges. We describe a biocompatible and biodegradable thermogelling polymer which provides an internal tamponade effect through surface tension and viscosity. Furthermore, we demonstrate its surgical efficacy in the repair of retinal detachment in a non-human primate model. By three-month post-surgery, the thermogel undergoes biodegradation, and is replaced by a vitreous-like body, thereby mimicking the biophysical needs of the natural vitreous body. In summary, we demonstrate the potential superiority of our thermogel compared to current vitreous tamponade gold standards; and present a significant advancement in the use of biomaterials for vitreo-retinal surgery.

2019-09-13 | PXD009525 | Pride

RNA-sequencing uncovers transcriptomic changes induced by a multiblock thermogelling polymer on healthy and disease-modeled retinal pigment epithelial cells

Project description:RNA-sequencing uncovers transcriptomic changes induced by a multiblock thermogelling polymer on healthy and disease-modeled retinal pigment epithelial cells

| PRJNA736472 | ENA

Protein composition of the subretinal fluid suggest selective diffusion of vitreous proteins in retinal detachment

Project description:Purpose: To study the proteome of the subretinal fluid (SRF) from rhegmatogenous retinal detachment (RRD) in search for novel markers for improved diagnosis and prognosis of RRD. Methods: Human undiluted SRF obtained during vitrectomy for primary RRD using a 41G needle (n = 24) was analyzed and compared to vitreous humor from 2-days post-mortem eyes (n = 20). Sample preparation underwent nanoflow liquid chromatography-tandem mass spectrometry (LC-MS/MS). Label-free quantification (LFQ) using MaxQuant was used to determine differential expressed proteins between SRF and vitreous humor. The intensity-based absolute quantification (iBAQ) was used to rank proteins according to their molar fractions within groups. Identification of proteins beyond the quantitative level was performed using the Mascot search engine. Results: The protein concentration of the control vitreous humor was lower and more consistent (1.2 ± 0.4 mg) than that of the SRF (17.9 ± 22 mg). The iBAQ analysis showed high resemblance between SRF and vitreous humor, except for crystallins solely identified in vitreous humor. The LFQ analysis found 37 protein misregulations between SRF and vitreous humor of which the blood coagulation pathway was found to be enriched using the PANTHER Classification System. Combined, the iBAQ, LFQ and Mascot analysis found an overlap only in chitinase-3-like protein 1 and galectin-3-binding protein unique to the SRF. Conclusions: The proteome of the SRF was highly represented by proteins involved in proteolysis. Such proteins can possibly serve as targets in modulating the effects of SRF in RD.

2020-10-20 | PXD019159 | Pride

Proteomic analysis of human vitreous humor

Project description:In this study, we investigated the proteome of normal human vitreous humor using high resolution Fourier transform mass spectrometry. We identified 1269 proteins. This largest catalog of vitreous proteins to date should facilitate biomedical research into pathological conditions of the eye including diabetic retinopathy, retinal detachment and cataract.

2014-08-08 | PXD000957 | Pride

The Multifaceted Role of Vitreous Hyalocytes: Orchestrating Inflammation, Angiomodulation, and Erythrophagocytosis in Proliferative Diabetic Retinopathy

Project description:Despite great advancements in proliferative diabetic retinopathy (PDR) therapy over the last decades, one third of treated patients continue to lose vision. Vitreous macrophages called hyalocytes have been implied in the pathogenesis of vitreoretinal proliferative disease previously but little is known about their role in PDR. In this study, we address molecular and cellular alterations of the PDR vitreous, in order to assess the potential contribution of hyalocytes to PDR development. A total of 55 patients were included in this study encompassing RNA-Sequencing analysis of vitreous hyalocytes from PDR and control patients, multiplex immunoassay and ELISA analysis of undiluted vitreous samples and isolation and immunohistochemical staining of cultured porcine hyalocytes. Our transcriptional analysis revealed an enhanced inflammatory signature of hyalocytes contributing to the cytokine pool within the PDR vitreous by expressing, among others, interleukin-6. The data further indicate an expression shift, involving angiopoietin-2, in PDR hyalocytes converting them to a proangiogenic cellular component, which may mediate formation of retinal neovascularization, hallmark of PDR. Finally, our data suggest a role of vitreous macrophages in erythrophagocytosis and, thereby, removal of vitreous hemorrhage, a severe complication of PDR. Immunomodulation of hyalocytes may thus prove an essential novel therapeutical approach in diabetic vitreoretinal disease.

2024-12-06 | GSE276892 | GEO

Comparative Proteomic Analysis of retro-oil fluid and vitreous fluid from patients

Project description:There have been reports of unexplained visual loss following intra-ocular silicone oil (SiO) tamponade in retinal detachment patients, yet the underlying mechanism is unknown. In the present study, we identified differentially expressed proteins in the vitreous humor of four groups (each group, n=3): pars plana vitrectomy (PPV) for rhegmatogenous retinal detachment (RD group), SiO removal after successful retinal reattachment (SO group), cataract surgery after successful retinal reattachment with sterilized air tamponade (FA group), and PPV for epiretinal membrane (ERM group). DIA was combined with two-dimensional liquid chromatography-tandem mass spectrometry (LC-MS/MS) to find expression changes in the proteome of vitreous.

2022-02-17 | PXD026816 | Pride

Protein profile of vitreous from Reeler model

Project description:To provide information about candidate factors contributing to impaired retinogenesis that might be useful tool for alternative therapeutic strategies, proinflammatory/profibrogenic mediator were evaluated in the vitreous from Reelin deficient mice, a model characterized by impaired retinogenesis. Vitreous samples from Reeler (n=9; RELN-/-) and WT (n=9; wild-type RELN+/+) mice at different postnatal (14, 21, 28) days, were used. Some potential candidate proteins were analyzed by chip array and confirmed by conventional analysis.

2019-02-13 | E-MTAB-7622 | biostudies-arrayexpress

miRNA profile in the vitreous of proliferative vitreoretinal disease patients

Project description:The microRNA profiles in the vitreous of proliferative vitreoretinal disease (PVD) such as proliferative diabetic retinopathy with fibrovascular membrane and macular hole (MH) patients were studied by RT-PCR. From each individual in the two cohorts: the PVD (n=3) and MH patients (n=3), vitreous specimens were collected and microRNAs were extracted for miRNA profiles analysis.

2015-11-02 | E-GEOD-74559 | biostudies-arrayexpress

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