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Transcriptomic profile of murine macrophage RAW264.7 in response to Salmonella flagellin

ABSTRACT: Transcriptomic profile of murine macrophage RAW264.7 in response to Salmonella flagellin

PROVIDER: PRJNA826837 | ENA |

REPOSITORIES: ENA

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Transcriptomic profile of murine macrophage RAW264.7 in response to Salmonella flagellin

Project description:Macrophages are an important component of the innate immune system. Flagellin has been extensively studied for its adjuvant activity owing to its TLR5 and NLRC4 binding sites. However, few studies have comprehensively investigated the effects of flagellin in macrophages using transcriptome sequencing. In this study, RNA sequencing (RNA-seq) was used to analyze the expression patterns of RAW264.7 cells induced by flagellin (FliC) of Salmonella typhimurium compared with unstimulated cells, to identify novel transcriptomic signatures in macrophages.

2022-12-31 | GSE200796 | GEO

GNPS Burkholderia thailandensis-exposed Murine Airway Epithelial and Murine Macrophage Cells and Monocultures

Project description:Extraction samples of murine airways epithelial LA-4 cells and murine macrophage RAW264.7 cells cultures exposed to different amounts of B. thailandensis for varying periods of time. Extracts of the adherent cells and their respective conditioned media were analyzed with LC-MS/MS to identify compounds detected within the aforementioned samples. Included are extracts of B. thailandensis monocultures, B. thailandensis-exposed LA-4 and RAW264.7 mammalian cells, and LA-4 and RAW264.7 unexposed monocultures.

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Host pathogen interaction between Staphylococcus aureus ATCC25923 and murine RAW264.7 macrophage-like cells

Project description:Host pathogen interaction between Staphylococcus aureus ATCC25923 and murine RAW264.7 macrophage-like cells

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Analysis of microRNA expression in murine BMDCs activated by flagellin

Project description:MicroRNAs (miRNAs) are small non-coding RNA molecules that regulate gene expression by either translational inhibition or mRNA degradation. However, the role of miRNAs in inflammation induced by flagellin (ligand of TLR5) has not been fully determined, Here, we identified differentially expressed miRNAs in murine bone marrow-derived dendritic cells (BMDCs) between flagellin (FliC) treatment and medium alone using miRNA microarray. And we further evaluated the role of miRNAs in inflammation induced by flagellin.

2021-11-13 | GSE188656 | GEO

Expression data from murine RAW264.7 macrophages

Project description:Expression data from murine RAW264.7 macrophages

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Botulinum neurotoxin A induced expression profiles in Raw264.7 cells

Project description:Botulinum neurotoxin type A (BoNT/A) is one of the most potent protein toxins, which makes it a possible biological weapon and therapeutic. Using microarray analysis we performed global transcriptional profiling of RAW264.7 cells, a murine alveolar macrophage cell line. Time dependent expression profiles after treatment of 1nM or 5nM Botulinum neurotoxin A

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Mass spectrometry-based proteomic exploration of diverse murine macrophage cellular models

Project description:Immortalised cell lines analogous to their primary cell counterparts are fundamental to research, particularly when large cell numbers are required. Here we report that immortalisation of bone marrow-derived macrophages using the J2-virus resulted in the loss of a protein of interest, MSR1, in wild-type cells by an unknown mechanism. This led us to perform an in-depth mass spectrometry-based proteomic characterisation of common murine macrophage cell lines (J774A.1, RAW264.7, and BMA3.1A7), with comparison to the immortalised bone marrow-derived macrophages (iBMDMs), as well as primary BMDMs. This revealed striking differences in protein profiles associated with macrophage polarisation, phagocytosis, pathogen recognition, and IFN signalling. J774A.1 cells were determined to be the most similar to the gold standard primary BMDM model, with BMA3.1A7 cells the least similar due to the reduction in abundance of several proteins related closely to macrophage function. This comprehensive proteomic data offers valuable insights into the selection of specific macrophage cell lines for cell signalling and inflammation research.

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flagellin

Project description:Flagellin diversity

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Characterization of the chemokine response of RAW264.7 cells to infection by murine norovirus

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Botulinum neurotoxin A induced expression profiles in Raw264.7 cells

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