Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ITS amplicon sequencing of the below-ground of grapevines in China

ABSTRACT: ITS amplicon sequencing of the below-ground of grapevines in China Raw sequence reads

PROVIDER: PRJNA831299 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR21992100_1.fastq.gz	Fastqsanger.gz
	SRR21992100_2.fastq.gz	Fastqsanger.gz
	SRR21992200.fastq.gz	Fastqsanger.gz
	SRR21992200_1.fastq.gz	Fastqsanger.gz
	SRR21992200_2.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 761

Similar Datasets

High-throughput 16S rRNA amplicon pyrosequencing of contaminated aquifer sediment

Project description:Contaminated aquifer (Dusseldorf-Flinger, Germany) templates extracted from 5 sediment depths ranging between 6.4 and 8.4 m below ground and over 3 years of sampling were amplified for amplicon pyrosequencing using the primers Ba27f (5’-aga gtt tga tcm tgg ctc ag-3’) and Ba519r (5’- tat tac cgc ggc kgc tg-3’), extended as amplicon fusion primers with respective primer A or B adapters, key sequence and multiplex identifiers (MID) as recommended by 454/Roche. Amplicons were purified and pooled as specified by the manufacturer. Emulsion PCR (emPCR), purification of DNA-enriched beads and sequencing run were performed following protocols and using a 2nd generation pyrosequencer (454 GS FLX Titanium, Roche) as recommended by the developer. Quality filtering of the pyrosequencing reads was performed using the automatic amplicon pipeline of the GS Run Processor (Roche), with a slight modification concerning the valley filter (vfScanAllFlows false instead of TiOnly) to extract the sequences. Demultiplexed raw reads were furhter trimmed for quality and lenght (>250 bp).

2012-11-11 | GSE25345 | GEO

High-throughput 16S rRNA amplicon pyrosequencing of contaminated aquifer sediment

2012-11-11 | E-GEOD-25345 | biostudies-arrayexpress

Below ground attack by root knot nematode Meloidogyne graminicola predisposes rice to blast disease

Project description:Magnaporthe oryzae (rice blast) and the root-knot nematode Meloidogyne graminicola are causing two of the most important pathogenic diseases jeopardizing rice production. Here, we show that root-knot nematode infestation on rice roots leads to important above-ground changes in plant immunity gene expression, which is correlated with significantly enhanced susceptibility to blast disease.

2018-11-02 | GSE89449 | GEO

RNAseq of leaves of tomato plants infected with a combination of four below- and above ground pathogens

Project description:RNAseq of leaves of tomato plants infected with a combination of four below- and above ground pathogens

| PRJEB54753 | ENA

Comparative proteomics of salt stressed own rooted and 110R grafted Thompson Seedless grapevines

Project description:Salt stress is a rising threat to agriculture system. The accumulation of salts near the plant roots hampers the normal uptake of water causing osmotic stress and ionic toxicity to the plant. Thompson Seedless is a popular table grape variety of Vitis vinifera L., which is sensitive to salt stress when grown on its own roots; grafting it onto a wild rootstock such as 110 Richtor (110R) makes it tolerant to salt stress. In the present study, shotgun-proteomics approach was used for the investigation of salt stress induced molecular response of own rooted and 110R grafted Thompson Seedless grapevines. A salt stress experiment was conducted on sixteen month old potted grapevines. The grapevines were treated with 150mM NaCl solution for seven days and the control vines were irrigated with tap water. The young leaf samples were collected from control and treated vines at three time-points viz. 6 hours, 48 hours and 7 days of salt stress. The stress responsive proteins identified through statistical analysis revealed a distinct response to salinity in both the vines.

2020-01-27 | PXD015515 | Pride

Expression data from 35S::VvCBF4-overexpressing grapevines

Project description:Overexpression of a grapevine C-repeat binding factor (CBF) gene, VvCBF4 in cv. Freedom was found to improve freezing survival in non-cold-acclimated vines. To understand the mechanistic basis of VvCBF4 transgene action, a transgenic line ('9-12') was genotyped using microarray-based mRNA expression profiling. Young grapevines (cv. 'Freedom') were grown in soil for RNA extraction and hybridization on Affymetrix microarrays. We sought to compare vines transformed with the VvCBF4 transcription factor, expressed under the 35S CaMV promoter (line '9-12') vs. an empty vector transformant (line '8-6'). We harvested the whole aerial tissues of four biological replicates, each.

2011-07-08 | E-GEOD-29948 | biostudies-arrayexpress

Ground water

Project description:Ground water Raw sequence reads

| PRJNA1059792 | ENA

Ground water

Project description:Ground water Raw sequence reads

| PRJNA1059800 | ENA

Ground water

Project description:Ground water Raw sequence reads

| PRJNA1059791 | ENA

Ground water

Project description:Ground water Raw sequence reads