Ontology highlight
ABSTRACT:
PROVIDER: PRJNA831625 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR18908059_1.fastq.gz | Fastqsanger.gz | |||
| SRR18908059_2.fastq.gz | Fastqsanger.gz | |||
| SRR18908060_1.fastq.gz | Fastqsanger.gz | |||
| SRR18908060_2.fastq.gz | Fastqsanger.gz | |||
| SRR18908061_1.fastq.gz | Fastqsanger.gz |
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Project description:Nematostella vectensis is a venomous organism with a complex life cycle. We harness different approaches to study venom composition dynamics along its life cycle. Our methods include but not limited to transcriptomica and proteomics. Here we perform semi quantitative analysis of Nematostella proteom at 4 developmental stages to compare abundance of different toxins.
2018-02-14 | PXD008218 | Pride
Project description:Changes in Nematostella vectensis proteome expression were analyzed in 2 different Nematostella populations along the east coast of USA in different stress conditions vrs. normal growth temperature.
2020-08-05 | PXD016943 | Pride
Project description:Nematostella is a sea anemone. Its venom ispacked into nematocysyts. In this work we studied proteom of the nematocyst soluble content from planula larva to identify new toxins.
2020-09-30 | PXD019085 | Pride
Project description:An animal’s ability to effectively capture prey and defend against predators is pivotal to its survival1. A key innovation in many predator-prey interactions is venom, which consists of many toxin proteins that shape its phenotype2. Its unusually direct relationship of gene-toxin-phenotype make it an appealing system for studies at the organismal level 3,4. In this work we use the sea anemone Nematostella vectensis as a model organism as it provides us with the opportunity to test for the first time how toxin-genotypes impact predator-prey interactions. Specifically, we compare a native-population5 and a transgenic line which both have significantly reduced levels of Nv1, the major toxin in adult Nematostella6, to animals with wildtype levels of Nv1. We demonstrate that the transgenic strain phenocopies native anemones lacking Nv1 as they both have impaired ability to defend themselves against grass shrimp, a native predator. Mummichog killifish, unexpectedly are attracted to Nematostella with wildtype-levels of Nv1, highlighting that Nv1 plays a complex role in shaping interspecific-interactions. Finally, we demonstrate an evolutionary tradeoff as the reduction of Nv1 levels causes faster growth and increased reproductive rates compared to Nematostella control lines. Overall, our results experimentally link organism’s venom to its physiology, reproduction and interspecific interactions.
2023-12-31 | PXD043447 | Pride
Project description:An animal’s ability to effectively capture prey and defend against predators is pivotal to its survival1. A key innovation in many predator-prey interactions is venom, which consists of many toxin proteins that shape its phenotype2. Its unusually direct relationship of gene-toxin-phenotype make it an appealing system for studies at the organismal level 3,4. In this work we use the sea anemone Nematostella vectensis as a model organism as it provides us with the opportunity to test for the first time how toxin-genotypes impact predator-prey interactions. Specifically, we compare a native-population5 and a transgenic line which both have significantly reduced levels of Nv1, the major toxin in adult Nematostella6, to animals with wildtype levels of Nv1. We demonstrate that the transgenic strain phenocopies native anemones lacking Nv1 as they both have impaired ability to defend themselves against grass shrimp, a native predator. Mummichog killifish, unexpectedly are attracted to Nematostella with wildtype-levels of Nv1, highlighting that Nv1 plays a complex role in shaping interspecific-interactions. Finally, we demonstrate an evolutionary tradeoff as the reduction of Nv1 levels causes faster growth and increased reproductive rates compared to Nematostella control lines. Overall, our results experimentally link organism’s venom to its physiology, reproduction and interspecific interactions.
2023-12-31 | PXD043484 | Pride
Project description:We generated ATAC-seq data for pre- and post-extraction venom gland samples and H3K4me3, H3K27ac, and CTCF ChIP-seq from post-extraction venom gland samples from the Prairie Rattlesnake to investigate patterns of chromatin accessibility, transcription factor binding, and insulation during venom production, and to identify open promoters and active enhancer regions.
2022-04-07 | GSE169217 | GEO
Project description:Background The generalist dipteran pupal parasitoid Nasonia vitripennis injects 79 venom peptides into the host before egg laying. This venom induces several important changes in the host, including developmental arrest, immunosuppression, and alterations to normal metabolism. It is hoped that diverse and potent bioactivities of N. vitripennis venom provide an opportunity for the design of novel acting drugs. However, currently very little is known about the individual functions of N. vitripennis venom peptides and less than half can be bioinformatically annotated. The paucity of annotation information complicates the design of studies that seek to better understand the potential mechanisms underlying the envenomation response. Although the RNA interference system of N. vitripennis provides an opportunity to functionally characterise venom encoding genes, with 79 candidates this represents a daunting task. For this reason we were interested in determining the expression levels of venom encoding genes in the venom gland, such that this information could be used to rank candidate venoms. To do this we carried out deep sequencing of the transcriptome of the venom gland and neighbouring ovary tissue and used RNA-seq to measure expression from the 79 venom encoding genes. The generation of a specific venom gland transcriptome dataset also provides further opportunities to investigate novel features of this highly specialised organ. Results High throughput sequencing and RNA-seq revealed that the highest expressed venom encoding gene in the venom gland was a serine protease called Nasvi2EG007167, which has previously been implicated in the apoptotic activity of N. vitripennis venom. As expected the RNA-seq confirmed that the N. vitripennis venom encoding genes are almost exclusively expressed in the venom gland relative to the neighbouring ovary tissue. Novel peptides appear to perform key roles in N. vitripennis venom function as only four of the highest 15 expressed venom encoding genes are bioinformatically annotationed. The high throughput sequencing data also provided evidence for the existence of an additional 471 novel genes in the Nasonia genome that are expressed in the venom gland and ovary. Finally, metagenomic analysis of venom gland transcripts identified viral transcripts that may play an important part in the N. vitripennis venom function. Conclusions The expression level information provided here for the 79 venom encoding genes provides an unbiased dataset that can be used by the N. vitripennis community to identify high value candidates for further functional characterisation. These candidates represent bioactive peptides that have value in drug development pipelines.
2016-12-31 | GSE76257 | GEO
Project description:We performed single-cell transcriptome analysis (using MARS-seq) in the cnidarian Nematostella vectensis. Additionally we performed bulk ATAC-seq experiments on wild-type specimens, as well as ELAV::mOrange reporter transgenic lines (separating ELAV+ and ELAV- cell populations, representing neurons and non-neurons, respectively).
2018-05-31 | GSE95723 | GEO
Project description:Mucuna pruriens extract MPE pretreatment may have a direct protective effect on heart (other than immunological neutralization of the venom neurotoxin and phospholipase A2 by the anti-MPE antibodies) that renders the heart more resistant to the toxic action of the venom The direct protective effect probably involves functional changes to the cardiac tissue that enable the heart to resist the reduction of contractility and rate induced by the cobra venom.To explore the possibility of the direct action of MPE pretreatment on heart and to understand the molecular events involved in the protection of MPE pretreatment against the lethal action of Naja sputatrix venom, gene expression studies were carried out using microarray analysis. Rats were divided into four groups (n=6): negative control (abbreviated as ‘negative’ group), MPE pretreated group (abbreviated as ‘MPE’ group), N. sputatrix venom-challenge group (abbreviated as ‘NS’ group) and N. sputatrix venom-challenge to MPE pretreated animals group (abbreviated as ‘MPE-NS’ group). In the ‘MPE’ group, rats were injected with MPE at a dose of 21 mg/kg (i.p.), on day 0, 7 and 14, and sacrificed on day 21. In the ‘negative’ group (the untreated, control group), rats were injected with saline of the same volume and sacrificed also on day 21. Hearts were then harvested immediately. In the N. sputatrix venom-challenge group (‘NS’ group), untreated rats were challenged with 1.5 LD50 (1.25 ?g/g) of N. sputatrix venom whereas in the venom challenge to MPE pretreated animals group (the ‘MPE-NS’ group), MPE pretreated rats were challenged with 1.5 LD50 (1.25 ?g/g) of N. sputatrix venom, both on day 21. For the ‘NS’ and ‘MPE-NS’ group, the rats were observed for 24 h after venom challenged and hearts were harvested as soon as death occurred or 24 h after the venom injection, whichever occurred first.
2014-12-22 | E-GEOD-46610 | biostudies-arrayexpress