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In vitro biocompatibility of ZrO2 vs Y-TZP alloys on Saos-2 cells: influence of composition and surface topography

ABSTRACT: In vitro biocompatibility of ZrO2 vs Y-TZP alloys on Saos-2 cells: influence of composition and surface topography

PROVIDER: PRJNA841478 | ENA |

REPOSITORIES: ENA

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In vitro biocompatibility of ZrO2 vs Y-TZP alloys on Saos-2 cells: influence of composition and surface topography

Project description:This study assess the biocompatibility of ZrO2 vs Y-TZP alloys on Saos-2 cells, through the combination of conventional toxicological assay, morphological observations and transcriptomic analysis performed on Saos-2 cells. The biocompatibility of ZrO2 alloys was evaluated in comparison with Y-TZP alloys, studying cell proliferation (WST-1) and cell morphology/adhesion (SEM) on human Saos-2 cell lines. Finally, an evaluation of gene expression was performed on Y-TZP alloys rough surface that seems more interesting regarding biocompatibility results.

2022-07-13 | GSE203572 | GEO

Saos-2 vs. Saos-2-2 cells

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In vitro biocompatibility of Ti-Cp vs Ti-26Nb alloys on Saos-2 and THP-1 cells

Project description:This study assess the biocompatibility of Ti-26Nb compared to Ti-Cp, a reference in implantology, through the combination of conventional toxicological assay, morphological observations and transcriptomic analysis performed on two different cells line : Saos-2 and THP-1. The biocompatibility of Ti-Cp implants was evaluated in comparison with Ti-26Nb implants both having a mirror polished surface, studying cell proliferation (trypan blue and WST-1) and cell morphology/adhesion (SEM) on human THP-1 and Saos-2 cell lines. Finally, an evaluation of a potential toxicity of potassium niobiate powder KNbO3 was carried out by measuring metabolic activity and realizing a transcriptomic study on the THP-1 line (the first one) to deepen the results observed with Ti-26Nb discs. Indeed, niobiate ions are potentially released from implant in cellular acidic environnement. Concerning Saos-2 cells, our results suggest that Ti-26Nb and Ti-Cp discs do not impact proliferation and viability. Concerning THP-1 cell line, a decrease in proliferation and viability is observed in contact of Ti-26Nb discs (hypothesis of a cell-line effect ?, no biocompatibility study on monocyte/macrophage cell lines such as THP-1 actually available in literature). KNbO3 powder does not impact viability of THP-1 cells and does not induce changes at molecular level. Taken together, these data suggest a possible toxicity of Ti-26Nb toward THP-1 cells not directly related to the niobium but perhaps to the manufacturing process of Titane niobium alloy.

2022-08-31 | GSE197987 | GEO

Saos-2 vs. Saos-2-2 cells

Project description:Saos-2-2, a TSG101-deficient stable line, was dereived from Saos-2 cells. The goal of this experiment was to determine the effects of TSG101 down-regulation on global gene expression. Saoa-2 and Saos-2-2 samples were labeled with Cys-3 and Cys-5 respectively, and hybrided with microarray simutaneously to differentiate the up- and down-regulated genes.

2013-10-11 | E-GEOD-50808 | biostudies-arrayexpress

Single-cell Analysis of Surface Topography-induced Foreign Body Response to Silicone Implants

Project description:Silicone-based medical devices are widely used in chronic implants and are generally perceived to be safe. However, immune-related complications including malignancies have recently been linked to textured breast implants. Here, we examine the influence of clinically approved breast implants surface features on host immune responses. Prosthetics with surface roughness of 0, 4, and 90 (Ra) were implanted in mammary fat pads of mice for 2 weeks and cells adjacent to the resulting tissue capsules were evaluated for foreign body immune responses using single-cell RNA-seq. Our findings identify a unique and finely tuned surface topography that is capable of modulating implant immunity to suppress foreign body response.

2021-05-28 | GSE164645 | GEO

Biocompatibility of ZrO<sub>2</sub> vs. Y-TZP Alloys: Influence of Their Composition and Surface Topography.

Project description:The osseointegration of implants is defined as the direct anatomical and functional connection between neoformed living bone and the surface of a supporting implant. The biological compatibility of implants depends on various parameters, such as the nature of the material, chemical composition, surface topography, chemistry and loading, surface treatment, and physical and mechanical properties. In this context, the objective of this study is to evaluate the biocompatibility of rough (Ra = 1 µm) and smooth (Ra = 0 µm) surface conditions of yttria-zirconia (Y-TZP) discs compared to pure zirconia (ZrO2) discs by combining a classical toxicological test, morphological observations by SEM, and a transcriptomic analysis on an in vitro model of human Saos-2 bone cells. Similar cell proliferation rates were observed between ZrO2 and Y-TZP discs and control cells, regardless of the surface topography, at up to 96 h of exposure. Dense cell matting was similarly observed on the surfaces of both materials. Interestingly, only 110 transcripts were differentially expressed across the human transcriptome, consistent with the excellent biocompatibility of Y-TZP reported in the literature. These deregulated transcripts are mainly involved in two pathways, the first being related to "mineral uptake" and the second being the "immune response". These observations suggest that Y-TZP is an interesting candidate for application in implantology.

| S-EPMC9267226 | biostudies-literature

In vitro biocompatibility of Ti-Cp vs Ti-26Nb alloys on Saos-2 and THP-1 cells

Project description:In vitro biocompatibility of Ti-Cp vs Ti-26Nb alloys on Saos-2 and THP-1 cells

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The effects of SMYD2 overexpression on ectopic RB in SAOS cells

Project description:Expression analysis of wild-type SAOS cells and SAOS cells transiently transfected with RB, SMYD2, or RB and SMYD2.

2011-01-01 | GSE22842 | GEO

Identification of target genes regulated by ΔNp63α in SaOS-2 cells stably expressed with ΔNp63α

Project description:To identify the target gene of ΔNp63α, in osteosarcoma cells, we performed the global gene expression profiling in SaOS- 2 cells stably overexpressing the ΔNp63α gene (SaOS- 2-ΔNp63α). ΔNp63α target genes were identified by comparison with genes expressed from SaOS-2-EV transfected with an empty vector ( SaOS-2-EV).

2014-02-27 | GSE54942 | GEO

DNase-seq profiling of Saos-2 and U2OS cell lines

Project description:We performed DNase-seq on two osteosarcoma cell lines, Saos-2 and U2OS. This was done to identify regulatory regions within the genomes of these cells. We performed hotspot calling on these data using Hotspot2 (https://github.com/Altius/hotspot2) to identify 51,556 DNaseI hypersensitive sites within Saos-2 cells and 63,388 within U2OS cells.

2020-01-03 | GSE142160 | GEO

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