Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcriptomic analysis of wild-type microglia isolated from fluorescent reporter mice

ABSTRACT: Transcriptomic analysis of wild-type microglia isolated from fluorescent reporter mice

PROVIDER: PRJNA842082 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR19389343_1.fastq.gz	Fastqsanger.gz
	SRR19389343_2.fastq.gz	Fastqsanger.gz
	SRR19389344_1.fastq.gz	Fastqsanger.gz
	SRR19389344_2.fastq.gz	Fastqsanger.gz
	SRR19389345_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 10

Similar Datasets

Transcriptomic analysis of wild-type microglia isolated from fluorescent reporter mice

Project description:Microglia isolated from tamoxifen-inducible Cx3cr1 cre mice harboring expression of a ZsGreen transgene in the ROSA26 locus were analyzed by RNA-sequencing. The purpose of this dataset is to serve as experimental controls as part of broader analyses of microglial-mediated neuroprotection during CNS infection with Toxoplasma gondii.

2022-06-30 | GSE204751 | GEO

Near-infrared fluorescent reporter stem cell model

Project description:Near-infrared fluorescent reporter stem cell model

| PRJEB51223 | ENA

Transcriptomic analysis of microglia and DC-like cells sorted using different reporter lines

Project description:We performed bulk transcriptomic analyses to compare the expression profile of different brain myeloid leukocytes. We used both Tg(p2ry12::p2ry12-GFP; cd45:DsRed) and Tg(mhc2dab:GFP; cd45:DsRed) adult fish, allowing to FACS-sort microglia identified in these animals as GFP+; DsRed+ or GFP+; DsRedlow cells, respectively. Brain putative DC-like cells were obtained using the Tg(mhc2dab:GFP; cd45:DsRed) reporter, and isolated as GFP+; DsRedhigh. These analyses confirm that cd45:DsRedhigh; mhc2dab:GFP+ cells share a similar transcriptome distinct from microglia and are DC-like cells.

2023-12-25 | E-MTAB-13228 | biostudies-arrayexpress

Effect of sex and brain region on gene expression within isolated microglia in adult C57Bl/6J mice.

Project description:We used mice that conditionally expressed a fluorescent reporter only in TMEM119 expressing cells to sort microglia for RNA-sequencing. We then performed gene expression profiling analysis using data obtained from RNA-seq of three different brain regions (prefrontal cortex, striatum, midbrain) in both male and female mice.

2022-07-27 | GSE203553 | GEO

iPS derived microglia like cells cultured with human neurons and astrocytes

Project description:In this article, Jose Polo and colleagues molecularly compare existing microglia differentiation methods from stem cells, generate a new dual fluorescent and enzymatic reporter tool to study microglia in vitro and in vivo and show that human astrocyte/neuron co-culture pushes the regulatory landscape of in vitro microglia-like cells to more closely resemble bona fide microglia.

2020-05-22 | GSE125872 | GEO

Identification of dormant cells using a novel fluorescent reporter of RNA-polymerase II activity

Project description:Identification of dormant cells using a novel fluorescent reporter of RNA-polymerase II activity

| PRJNA612818 | ENA

Color-flu: multi-spectral fluorescent reporter influenza viruses as powerful tools for in vivo studies

Project description:We sorted macrophages (known to be infected by influenza viruses from the lungs of mice infected with MA-Venus-PR8 on the basis of their fluorescent protein expression and performed microarray analysis. Macrophages isolated from the lungs of mice inoculated with PBS (naive macrophages) served as controls.

2014-12-24 | GSE64473 | GEO

Acutely isolated murine cortical astrocytes and microglia: Alzheimer's disease vs wildtype

Project description:Isolation of glia from Alzheimer's mice reveals inflammation andÂ dysfunction. Reactive astrocytes and microglia are associated with amyloid plaques in Alzheimer's disease (AD). Yet, not much is known about the molecular alterations underlying this reactive phenotype. To get an insight into the molecular changes underlying AD induced astrocyte and microglia reactivity, we performed a transcriptional analysis on acutely isolated astrocytes and microglia from the cortex of aged controls and APPswe/PS1dE9 AD mice. As expected, both cell types acquired a proinflammatory phenotype, which confirms the validity of our approach. Interestingly, we observed that the immune alteration in astrocytes was relatively more pronounced than in microglia. Concurrently, our data reveal that astrocytes display a reduced expression of neuronal support genes and genes involved in neuronal communication. The microglia showed a reduced expression of phagocytosis and/or endocytosis genes. Co-expression analysis of a human AD expression data set and the astrocyte and microglia data sets revealed that the inflammatory changes in astrocytes were remarkably comparable in mouse and human AD, whereas the microglia changes showed less similarity. Based on these findings we argue that chronically proinflammatory astrocyte and microglia phenotypes, showing a reduction of genes involved in neuronal support and neuronal signaling, are likely to contribute to the neuronal dysfunction and cognitive decline in AD. 2 cell types from 2 conditions: cortical microglia and cortical astrocytes from 15-18 month old APPswe/PS1dE9 mice compared to wildtype littermates. Biological replicates: microglia from APPswe/PS1dE9, N=7, microglia from WT, N=7, astrocytes from APPswe/PS1dE9, N=4, microglia from WT, N=4

2015-11-04 | E-GEOD-74615 | biostudies-arrayexpress

RNA sequencing data of microglia isolated from brains of WT, Hexb-tdTomato, Hexb-CreERT2 and Hexb-KO mice. {Reporter]

Project description:RNA sequencing of FACS sorted microglia isolated from brain tissue of WT, Hexb-tdTomato, Hexb-CreERT2 and Hexb-KO mice was performed to test for potential differences in gene expression caused by the knock-in of tdTomato and CreERT2 into the Hexb locus.

2020-05-25 | GSE148412 | GEO

mCherry contains a fluorescent protein isoform that interferes with its reporter function

Project description:Fluorescent proteins are essential reporters in cell biology and molecular biology. Here, we reveal that red-fluorescent proteins possess an alternative translation initiation site that produces a short functional protein isoform. The short isoform creates significant background fluorescence that biases the outcome of expression studies. Our investigation identifies the short protein isoform, traces its origin, and determines the extent of the issue within the family of red fluorescent protein. Our analysis shows that the short isoform defect of the red fluorescent protein family may affect the interpretation of many published studies. Finally, we provide a re-engineered mCherry variant that lacks background expression as an improved tool for imaging and protein expression studies.

2022-04-19 | PXD032954 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data