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RNA-sequencing of brain mural cells differentiated from human pluripotent stem cell-derived neural crest via Notch3 activation

ABSTRACT: RNA-sequencing of brain mural cells differentiated from human pluripotent stem cell-derived neural crest via Notch3 activation

PROVIDER: PRJNA844106 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR19461373_1.fastq.gz	Fastqsanger.gz
	SRR19461373_2.fastq.gz	Fastqsanger.gz
	SRR19461374_1.fastq.gz	Fastqsanger.gz
	SRR19461374_2.fastq.gz	Fastqsanger.gz
	SRR19461375_1.fastq.gz	Fastqsanger.gz

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Notch3 ChIP-sequencing of brain mural cells differentiated from human pluripotent stem cell-derived neural crest via Notch3 activation

Project description:Molecular mechanisms underlying the differentiation of brain mural cells from neural crest are poorly understood. We found that activation of Notch3 signaling in human pluripotent stem cell-derived neural crest (using lentiviral overexpression of the human Notch3 intracellular domain, N3ICD) was sufficient to direct the differentiation of mural cells. We used Notch3 ChIP-sequencing to identify cis-regulatory elements directly bound by the Notch3 transcriptional activation complex in this system.

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RNA-sequencing of brain mural cells differentiated from human pluripotent stem cell-derived neural crest via Notch3 activation

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Brain mural cells differentiated from human pluripotent stem cell-derived neural crest via Notch3 activation

Project description:Brain mural cells differentiated from human pluripotent stem cell-derived neural crest via Notch3 activation

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Notch3 ChIP-sequencing of brain mural cells differentiated from human pluripotent stem cell-derived neural crest via Notch3 activation

Project description:Notch3 ChIP-sequencing of brain mural cells differentiated from human pluripotent stem cell-derived neural crest via Notch3 activation

| PRJNA844105 | ENA

Notch3 directs differentiation of brain mural cells from human pluripotent stem cell-derived neural crest.

Project description:Brain mural cells regulate development and function of the blood-brain barrier and control blood flow. Existing in vitro models of human brain mural cells have low expression of key mural cell genes, including NOTCH3. Thus, we asked whether activation of Notch3 signaling in hPSC-derived neural crest could direct the differentiation of brain mural cells with an improved transcriptional profile. Overexpression of the Notch3 intracellular domain (N3ICD) induced expression of mural cell markers PDGFRβ, TBX2, FOXS1, KCNJ8, SLC6A12, and endogenous Notch3. The resulting N3ICD-derived brain mural cells produced extracellular matrix, self-assembled with endothelial cells, and had functional KATP channels. ChIP-seq revealed that Notch3 serves as a direct input to relatively few genes in the context of this differentiation process. Our work demonstrates that activation of Notch3 signaling is sufficient to direct the differentiation of neural crest to mural cells and establishes a developmentally relevant differentiation protocol.

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Brain mural cells differentiated from human pluripotent stem cell-derived neural crest via Notch3 activation

Project description:This SuperSeries is composed of the SubSeries listed below.

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Embryonic and adult neural crest stem cells

Project description:Cells were isolated from mouse embryonic neural crest stem cells at culture day 2 (NCSC), from day 7 in vitro differentiated progeny (NCP) and day 2 epidermal neural crest stem cells from bulge explants of adult whisker follicles (EPI-NCSC). Keywords: LongSAGE embryonic neural crest stem cells at culture day 2 (NCSC), from day 7 in vitro differentiated progeny (NCP) and day 2 epidermal neural crest stem cells from bulge explants of adult whisker follicles (EPI-NCSC).

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