Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ABSTRACT: Transcriptome in normal colonic epithelia of young (2-month-old) and aged (18-month-old) mice

PROVIDER: PRJNA848952 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR19646052_1.fastq.gz	Fastqsanger.gz
	SRR19646052_2.fastq.gz	Fastqsanger.gz
	SRR19646053_1.fastq.gz	Fastqsanger.gz
	SRR19646053_2.fastq.gz	Fastqsanger.gz
	SRR19646054_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 12

Similar Datasets

Suppression of inflammaging prolongs hematopoietic healthspan [dataset 5]

Project description:The impact of Thbs1 gene deletion on BMEC activity during physiological aging is unknown. In this experiment, RNA Seq analysis was performed on BMECs derived from aged (18 month-old) Thbs1 knockout mice. BMECs derived from young (3 month-old) and aged (18 month-old) control mice were used as controls for comparison.

2025-01-03 | GSE255654 | GEO

Suppression of inflammaging prolongs hematopoietic healthspan [dataset 4]

Project description:The impact of Thbs1 gene deletion on BM MSC activity during physiological aging is unknown. In this experiment, RNA Seq analysis was performed on BM MSCs derived from aged (18 month-old) Thbs1 knockout mice. MSCs derived from young (3 month-old) and aged (18 month-old) control mice were used as controls for comparison.

2025-01-03 | GSE255653 | GEO

Suppression of inflammaging prolongs hematopoietic healthspan [dataset 3]

Project description:The impact of Thbs1 gene deletion on HSC activity during physiological aging is unknown. In this experiment, RNA Seq analysis was performed on BM HSCs derived from aged (18 month-old) Thbs1 knockout mice. HSCs derived from young (3 month-old) and aged (18 month-old) control mice were used as controls for comparison.

2025-01-03 | GSE255652 | GEO

Proteomic characterizationof the circle of Willis during aging

Project description:The Circle of Willis (CoW), the ring of arteries at the base of the brain, links the intracerebral arteries to one another to maintain adequate cerebral perfusion. The CoW proteome is affected in cerebrovascular and neurodegenerative diseases, but changes related to aging have not been described. Here, we report on a quantitative proteomics analysis comparing the CoW from five young (two-three-month-old) and five aged (18–20-month-old) mice.

2024-06-26 | PXD043001 | Pride

Age of the bone marrow dictates clonality of smooth muscle-derived cells in the atherosclerotic plaque [bulk RNA-seq]

Project description:Purpose: The goal of this study is to investigate the gene expression in aged (18 month old) and young (3 month old) bone marrow-derived monocytes. Methods: Bone marrow was harvested by flushing the femurs and tibias of young (3 month old) and aged (18 month old) mice in PBS with 2% fetal bovine serum (FBS) on ice. Cells were isolated from bone marrow by density gradient centrifugation using Lympholyte (Cedarlane, CL0531). After centrifugation at 1300 g for 20 min, bone marrow cells were collected from the interface and washed in Ca2+/Mg2+ - free HBSS. The cells were dissociated to a single-cell suspension by filtering through a 70-µm nylon mesh. For monocyte isolation, a suspension of bone marrow cells was stained with Alexa 700 anti-CD3 (1:100, BioLegend, 100215), PE-Cy7 anti-CD19 (1:100, BioLegend, 115520), FITC anti-CD11b (1:100, BD Pharmingen, 553310) and APC anti-Ly6c antibodies (1:100, BioLegend, 128015) in PBS with 0.5% FBS for 30 min. CD11b+Ly6c+CD3-CD19- monocytes were isolated with a BD FACSAria cell sorter. Total RNA was extracted and subjected to bulk RNA-seq using Illumina HiSeq 2000. Results: Expression profiles of the transcriptome are compared between bone marrow-derived monocytes isolated from aged (18 month old) and young (3 month old) mice. Conclusions: Significant differences in the expression levels of genes are noted between the two age groups.

2022-08-01 | GSE206429 | GEO

Disruption of the Circadian Rhythm Is Exacerbated by Aging and Partially Restored by Time-restricted Feeding

Project description:To characterize any differences in the effects of Circadian rhythm disorders (CRD) on mice of various ages, we selected young (8-week-old), and aged (18-month-old) mice and created a model of CRD by modifying the light/dark cycle under which they were housed.

2024-05-09 | GSE264350 | GEO

MicroRNA-29 in Aortic Dilation: Implications for Aneurysm Formation

Project description:We compared the aorta of 6-weeks-old mice (young) with 18-months-old mice (old). Using the publicly available tools Sylamer and DIANA-mirExTra, we identified an enrichment for miR-29 binding sites in the 3'UTR of genes downregulated in the aged aortas. We subsequently showed that inhibition of miR-29 in aged mice prevented dilation of the aorta. aortas of 6 week old and 18 month old mice

2011-10-13 | E-GEOD-32937 | biostudies-arrayexpress

5-Hydroxymethylcytosine profiling of aging hippocampi

Project description:We profiled 5-hydroxymethylcytosine patterns in hippocampi using hydroxymethylated DNA immunoprecipitation followed by Illumina deep sequencing (hMeDIP-Seq). We profiled the hippocampi of 4 young (3 month old) and 4 aged (18 month old) C57BL/6 mice. Additionally, we profiled the 5-hmC patterns of hippocampi that were stereotactically injected with lentiviral constructs that overexpressed Tet2 or luciferase.

2018-02-25 | GSE102473 | GEO

Transcriptomic analysis of mouse brain microglia on aging-associated changes in response to cerebral ischemia

Project description:Senescence-associated alterations in microglia may have profound impact on cerebral homeostasis and stroke outcomes. However, the lack of a transcriptome-wide comparison between young and aged microglia in the context of ischemia limits our understanding of aging-related mechanisms. Herein, we performed bulk RNA sequencing analysis of microglia purified from cerebral hemispheres of young adult (10-week-old) and aged (18-month-old) mice 5 days after distal middle cerebral artery occlusion or sham operation. Considerable transcriptional differences were observed between young and aged microglia in healthy brains, indicating heightened chronic inflammation in aged microglia. Following stroke, the overall transcriptional activation was more robust in young microglia than in aged microglia. Gene clusters with functional implications in immune inflammatory responses, immune cell chemotaxis, tissue remodeling, and cell-cell interactions were markedly activated in microglia of young but not aged stroke mice. These alterations in microglial gene response may contribute to aging-driven vulnerability and poorer recovery after ischemic stroke.

2020-05-31 | GSE145265 | GEO

Single cell RNA sequencing of myeloid cells from lungs of young and aged mice

Project description:Single cell RNA sequencing from lungs of young (2-3 month old) and aged (20-22 month old) mice.

2021-10-20 | GSE167236 | GEO