Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcriptomics of Toxoplasma gondii sporulating oocysts

ABSTRACT: Transcriptomics of Toxoplasma gondii sporulating oocysts

PROVIDER: PRJNA850353 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR19733948.fastq.gz	Fastqsanger.gz
	SRR19733949.fastq.gz	Fastqsanger.gz
	SRR19733950.fastq.gz	Fastqsanger.gz
	SRR19733951.fastq.gz	Fastqsanger.gz
	SRR19733952.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 6

Similar Datasets

Transcriptomics of Toxoplasma gondii sporulating oocysts

Project description:Toxoplasma gondii is an apicomplexan parasite that approximately infects one third of the human population worldwide. When sporulated oocysts that are shed in the faeces of infected felids are accidentally ingested through contaminated food or water, sporozoites can infect the intestine of the intermediate host. Upon this infection, the fast replicating tachyzoites then disseminate overall the body and can cross the blood-brain, blood-retina or the blood-placenta barrier. Eventually, immune pressure will lead to differentiation into the slow replicating bradyzoites that are contained within tissue cysts for life. Despite the importance of sporulation for transmission, the process is not yet understood. We therefore exploited Illumina Next-Generation RNA-Sequencing to analyse the transcriptome of oocysts in the process of sporulation and compared it to unsporulated and sporulated oocysts. We thus identified genes that are highly specific to the sporulation process and that may be involved in the formation of sporozoites.

2022-06-20 | GSE206344 | GEO

Gene expression analysis of Hammondia hammondi and Toxoplasma gondii sporulated oocysts

2014-10-02 | GSE61963 | GEO

Gene expression analysis of Hammondia hammondi and Toxoplasma gondii sporulated oocysts

Project description:To date little is known about the transcriptome of Hammondia hammondi, the nearest extant relative of Toxoplasma gondii. In this study we used an existing microarray to query Toxoplasma gondii and Hammondia hammondi transcript abundance in sporulated oocysts. Oocysts of the VEG strain of Toxoplasma gondii, and the HhCatGer041 strain of Hammondia hammondi, were isolated from cat feces by sucrose flotation, and sporulated for ~3-6 months in 2% sulfuric acid. RNA was isolated from Bleach-treated oocyst preparations using the Trizol reagent. RNA was biotinylated for hybridization to Toxo 169 Affymetrix chips using the Illumina Total Prep RNA labeling kit (Ambion). For each species 3 separate RNA isolations were performed on the same batch of oocysts and hybridized to individual microarrays.

2014-10-02 | E-GEOD-61963 | biostudies-arrayexpress

Transcriptomics of Toxoplasma gondii enteroepithelial stages

Project description:Toxoplasma gondii is a zoonotic pathogen for which felids serve as definitive hosts. In cats, the parasite undergoes several rounds of asexual replication before entering the sexual cycle which gives rise to oocysts that are shed into the environment. These then sporulate and become infective to humans and live stock. To understand the genes involved in the parasite development in the felid host and identify potential intervention targets, we designed a transcriptomic approach to compare the cat intestinal stages with the well characterised tachyzoites that mediate acute infection and tissue cysts that are responsible for chronic infection. Cats were infected with T. gondii tissue cysts from mouse brain and sampled the intestinal stages at day 3, 5 and 7 post infection. As an input sample, we also collected tissue cysts from mouse brain as well as in vitro cultivated tachyzoites. Total RNA was extracted, enriched for mRNA and used for cDNA synthesis. RNA-Seq was then performed to describe the transcriptomic repertoire of each time point/life cycle stage.

2018-03-30 | GSE108740 | GEO

Transcriptomics of Toxoplasma gondii enteroepithelial stages

Project description:Transcriptomics of Toxoplasma gondii enteroepithelial stages

| PRJNA428456 | ENA

Gene expression analysis of Hammondia hammondi and Toxoplasma gondii sporulated oocysts

Project description:Gene expression analysis of Hammondia hammondi and Toxoplasma gondii sporulated oocysts

| PRJNA262816 | ENA

Toxoplasma gondii

Project description:Toxoplasma gondii

| PRJDA33425 | ENA

Toxoplasma gondii RNASeq

Project description:Recent advances in high throughput sequencing methodologies allow the opportunity to probe in depth the transcriptomes of organisms including N. caninum and Toxoplasma gondii. In this project, we are using Illumina sequencing technology to analyze the transcriptome (RNA-Seq) of experimentally accessible stages (e.g. tachyzoites at different times points) of T. gondii VEG strain. The aim is to make comparative transcriptional landscape maps of Neospora and Toxoplasma at different time points at different life cycle stages and compare levels of expression of orthologous genes in these two organisms.

2011-02-08 | E-MTAB-550 | biostudies-arrayexpress

Expression QTL mapping of Toxoplasma gondii genes

Project description:This SuperSeries is composed of the following subset Series: GSE11437: Expression QTL mapping of Toxoplasma gondii genes, Bradyzoite array GSE11514: Expression QTL mapping of Toxoplasma gondii genes, Tachyzoite array Keywords: SuperSeries Refer to individual Series

2008-05-22 | E-GEOD-11515 | biostudies-arrayexpress

Toxoplasma gondii

Project description:Toxoplasma gondii Genome sequencing

| PRJNA258152 | ENA